| pubmed-article:7115808 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C0036226 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C0031715 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C0486805 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C1710236 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C0456962 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:7115808 | lifeskim:mentions | umls-concept:C0205227 | lld:lifeskim |
| pubmed-article:7115808 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:7115808 | pubmed:dateCreated | 1982-12-2 | lld:pubmed |
| pubmed-article:7115808 | pubmed:abstractText | Separation of phosphorylated sarcoplasmic reticulum (SR) fragments by polyacrylamide gel disc electrophoresis in the presence of Na-DS revealed that the radioactivity is distributed in protein zones with molecular weights of 95,000 and 6000-8000. The phosphorylation of the protein with m. w. of 95,000 is Ca2+-dependent. The tryptic hydrolysis of the phosphorylated SR fragments from fast skeletal muscles results in a loss of radioactivity by 60-70%; phospholipase C from Clostridium welchii reduces the labelled phosphate content by 40-50%. The cAMP-dependent protein kinase inhibitor decreases the phosphorylation of both substrates. The substrate of phosphorylation with m. w. of 6000-8000 is not stained with Amidoschwartz 10B or Coumassie brilliant blue. Extraction by an acidified chlorophorm--methanol mixture results in a proteolipid with specific radioactivity exceeding that of the original preparation of phosphorylated SR membranes 3-4-fold. Thin-layer chromatography on Silufol plates and Silicagel KSK showed that the proteolipid is not chromatographically homogeneous after 2-fold precipitation by diethyl ether and is localized in a band with Rf varying from 0.6 to 0.8. The fluorescence spectrum of the proteolipid in a chlorophorm--methanol--HCl solution is represented by an assymmetrical structure-free band with a maximum at 350 nm. A possible role of phosphorylase b and proteolipid in manifestation of the functional activity of the SR fragments is discussed. | lld:pubmed |
| pubmed-article:7115808 | pubmed:language | rus | lld:pubmed |
| pubmed-article:7115808 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7115808 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7115808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7115808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7115808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7115808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7115808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7115808 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7115808 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7115808 | pubmed:issn | 0320-9725 | lld:pubmed |
| pubmed-article:7115808 | pubmed:author | pubmed-author:Kurski?M DMD | lld:pubmed |
| pubmed-article:7115808 | pubmed:author | pubmed-author:KondratiukT... | lld:pubmed |
| pubmed-article:7115808 | pubmed:author | pubmed-author:LitvinenkoE... | lld:pubmed |
| pubmed-article:7115808 | pubmed:author | pubmed-author:FedorovA NAN | lld:pubmed |
| pubmed-article:7115808 | pubmed:author | pubmed-author:MeshkovaL ILI | lld:pubmed |
| pubmed-article:7115808 | pubmed:author | pubmed-author:OsipenkoA AAA | lld:pubmed |
| pubmed-article:7115808 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7115808 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:7115808 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7115808 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7115808 | pubmed:pagination | 950-6 | lld:pubmed |
| pubmed-article:7115808 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:meshHeading | pubmed-meshheading:7115808-... | lld:pubmed |
| pubmed-article:7115808 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:7115808 | pubmed:articleTitle | [Characterization of endogenous phosphorylation substrates of sarcoplasmic reticulum fragments from fast skeletal muscles of the rabbit]. | lld:pubmed |
| pubmed-article:7115808 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7115808 | pubmed:publicationType | English Abstract | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7115808 | lld:pubmed |
