| pubmed-article:7114248 | pubmed:abstractText | Considerable difficulty has been encountered in defining the physiological significance of sodium tracer kinetic measurements in cardiac muscle. In this study, 24Na+ efflux experiments were performed by directly monitoring tissue radioactivity during the superfusion of growth-oriented embryonic chick heart cells in tissue cultured. The cellular 24Na+ efflux from contractile preparations exhibited at least two exponential components whereas noncontractile, fibroblastlike preparations had a single efflux component similar in rate to the slower component of the contractile preparations. We concluded that the slow component represents efflux from nonmuscle cells, whereas the faster component reflects the muscle cell compartment. The mean Na+ efflux rate constants for contractile preparations (beating 150 min-1) were 3.1 and 0.35 min-1. Intracellular Na+ concentrations, as determined by isotope uptake and by flame photometry, were 18 and 16 mM for contractile and nonmuscle preparations, respectively. The steady-state, transmembrane fluxes are 98 and 5 pmol . cm-2 . s-1 for muscle and nonmuscle cells, respectively. The Na+ efflux kinetics in 10(-4) M ouabain were reduced by approximately 16% from the control value. These findings indicate that the greater part of the steady-state Na+ efflux in cultured heart cells is due to mechanisms other than the Na+-K+ pump. | lld:pubmed |
