| pubmed-article:7050914 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C0917792 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C1708632 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C1522485 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C0684192 | lld:lifeskim |
| pubmed-article:7050914 | lifeskim:mentions | umls-concept:C0185027 | lld:lifeskim |
| pubmed-article:7050914 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:7050914 | pubmed:dateCreated | 1982-10-29 | lld:pubmed |
| pubmed-article:7050914 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7050914 | pubmed:abstractText | We describe an in vitro protocol for quickly generating overlapping terminal-labeled restriction fragments for DNA sequence analysis via the Maxam-Gilbert technique. The protocol involves introducing mercurated nucleotides into one end of a region to be sequenced, partial digestion with several restriction enzymes and terminal-labeling, separation of the mercurated restriction enzymes and terminal-labeling, separation of the mercurated restriction fragments from non-mercurated ones on a thiol column and resolution of the different mercurated fragments on one preparative agarose gel. The protocol was used to determine the nucleotide sequence of a 980 base pair cDNA that contains the coding region for a variable surface glycoprotein of Trypanosoma brucei. It could just as quickly and easily be used to obtain many terminal-labeled overlapping restriction fragments covering a region of several kilobases. | lld:pubmed |
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| pubmed-article:7050914 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7050914 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7050914 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7050914 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7050914 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:7050914 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:7050914 | pubmed:author | pubmed-author:ChenK KKK | lld:pubmed |
| pubmed-article:7050914 | pubmed:author | pubmed-author:DonelsonJ EJE | lld:pubmed |
| pubmed-article:7050914 | pubmed:author | pubmed-author:HartleyJ LJL | lld:pubmed |
| pubmed-article:7050914 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7050914 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:7050914 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:7050914 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7050914 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7050914 | pubmed:pagination | 4009-25 | lld:pubmed |
| pubmed-article:7050914 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7050914 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:7050914 | pubmed:articleTitle | A mercury-thiol affinity system for rapid generation of overlapping labeled DNA fragments for DNA sequencing. | lld:pubmed |
| pubmed-article:7050914 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7050914 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7050914 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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