| pubmed-article:7045875 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7045875 | lifeskim:mentions | umls-concept:C0018557 | lld:lifeskim |
| pubmed-article:7045875 | lifeskim:mentions | umls-concept:C0021270 | lld:lifeskim |
| pubmed-article:7045875 | lifeskim:mentions | umls-concept:C0553257 | lld:lifeskim |
| pubmed-article:7045875 | lifeskim:mentions | umls-concept:C0010853 | lld:lifeskim |
| pubmed-article:7045875 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:7045875 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:7045875 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:7045875 | pubmed:dateCreated | 1982-8-14 | lld:pubmed |
| pubmed-article:7045875 | pubmed:abstractText | A monoclonal antibody directed against eukaryotic mRNA 5'-cap-binding protein (anti-CBP antibody) was used to localize cap-binding protein (CBP) in BHK-21 baby hamster kidney cells by immunofluorescence microscopy. It was found that the antibody reacts with a fibrous network extending through the cytoplasm in a radial arrangement. The network behaves like intermediate filaments in colchicine-treated cells, suggesting a direct or indirect linkage of CBP with intermediate filaments. The association of CBP with a cytoskeletal element was further confirmed by isolation of proteins from Triton X-100-extracted cells and identification of CBP in the cytoskeletal fraction with anti-CBP antibody. The major polypeptide reacting with anti-CBP antibody is a Mr 50,000 component. Tryptic peptide mapping showed that this polypeptide is related to a Mr 24,000 polypeptide identified as CBP in earlier experiments [Sonenberg, N., Morgan, M. A., Testa, D., Colonna, R. J. & Shatkin, A. J. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847]. | lld:pubmed |
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| pubmed-article:7045875 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7045875 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7045875 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7045875 | pubmed:month | May | lld:pubmed |
| pubmed-article:7045875 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:7045875 | pubmed:author | pubmed-author:TrachselHH | lld:pubmed |
| pubmed-article:7045875 | pubmed:author | pubmed-author:ZumbéAA | lld:pubmed |
| pubmed-article:7045875 | pubmed:author | pubmed-author:StähliCC | lld:pubmed |
| pubmed-article:7045875 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7045875 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:7045875 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7045875 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7045875 | pubmed:pagination | 2927-31 | lld:pubmed |
| pubmed-article:7045875 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7045875 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:7045875 | pubmed:articleTitle | Association of a Mr 50,000 cap-binding protein with the cytoskeleton in baby hamster kidney cells. | lld:pubmed |
| pubmed-article:7045875 | pubmed:publicationType | Journal Article | lld:pubmed |
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