| pubmed-article:6999873 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6999873 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:6999873 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:6999873 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:6999873 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6999873 | lifeskim:mentions | umls-concept:C1264633 | lld:lifeskim |
| pubmed-article:6999873 | pubmed:dateCreated | 1980-12-18 | lld:pubmed |
| pubmed-article:6999873 | pubmed:abstractText | A modified pronase digestion procedure is described for isolating nonparenchymal liver cells from vitamin A treated rats, which yielded a 15-23% population of lipocytes in the total cell suspension. The criteria for defining a lipocyte was the appearance of vitamin A containing cells as determined by fluorescent microscopy. Measurement of alcohol dehydrogenase and retinol dehydrogenase activities indicated that these enzyme activities were not present in the isolated nonparenchymal cells. A lipocyte-rich fraction of nonparenchymal cells was obtained by centrifugation of purified nonparenchymal cells in a linear Metrizamide gradient. Vitamin A fluorescence and chemical assay of vitamin A in the cell fractions indicated a four-fold enrichment of lipocytes in the cell fraction with d = 1.043 g/ml. Fractions high in vitamin A also had numerous cells with fat droplets as shown by transmission electron microscopy. | lld:pubmed |
| pubmed-article:6999873 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6999873 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6999873 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6999873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6999873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6999873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6999873 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6999873 | pubmed:issn | 0065-2598 | lld:pubmed |
| pubmed-article:6999873 | pubmed:author | pubmed-author:VeechR LRL | lld:pubmed |
| pubmed-article:6999873 | pubmed:author | pubmed-author:OttoD ADA | lld:pubmed |
| pubmed-article:6999873 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6999873 | pubmed:volume | 132 | lld:pubmed |
| pubmed-article:6999873 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6999873 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6999873 | pubmed:pagination | 509-17 | lld:pubmed |
| pubmed-article:6999873 | pubmed:dateRevised | 2008-10-10 | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:meshHeading | pubmed-meshheading:6999873-... | lld:pubmed |
| pubmed-article:6999873 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6999873 | pubmed:articleTitle | Isolation of a lipocyte-rich fraction from rat liver nonparenchymal cells. | lld:pubmed |
| pubmed-article:6999873 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6999873 | pubmed:publicationType | In Vitro | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6999873 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6999873 | lld:pubmed |
