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pubmed-article:6996828pubmed:abstractTextA method was developed that allows the analysis of neuropeptides and monoamines in a single tissue section by the application of the unlabeled antibody method for peptide staining to tissue sections freeze-dried for formaldehyde-induced monoamine histofluorescence. The hypothalamic magnocellular system of male albino rats served as a model for this study; neurons were stained with anti-neurophysin sera, which mark the vasopressin- and oxytocin-associated proteins. Neurophysin-containing perikarya appeared to be surrounded by catecholamine-containing varicosities. This phenomenon was seen to varying degrees within the supraoptic and paraventricular nuclei. The juxtaposition of varicosities and peptidergic neurons suggests an afferent fiber-target neuron relationship that might favor a functional interaction between monoamines and neuropeptides.lld:pubmed
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pubmed-article:6996828pubmed:authorpubmed-author:SladekJ RJRJrlld:pubmed
pubmed-article:6996828pubmed:authorpubmed-author:McNeillT HTHlld:pubmed
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pubmed-article:6996828pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:6996828pubmed:year1980lld:pubmed
pubmed-article:6996828pubmed:articleTitleSimultaneous monoamine histofluorescence and neuropeptide immunocytochemistry. IV. Verification of catecholamine-neurophysin interactions through single-section analysis.lld:pubmed
pubmed-article:6996828pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:6996828pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed