| pubmed-article:6969276 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0699040 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0205208 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0123263 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0034580 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0205390 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C2702333 | lld:lifeskim |
| pubmed-article:6969276 | lifeskim:mentions | umls-concept:C0205352 | lld:lifeskim |
| pubmed-article:6969276 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6969276 | pubmed:dateCreated | 1981-2-24 | lld:pubmed |
| pubmed-article:6969276 | pubmed:abstractText | The aim of this work was to detect Fc gamma receptors on human lymphoblastoid cells using a solid phase radioimmunoassay (RIA), specific to human IgG. RIA was performed by coupling rabbit anti-human IgG (AHIgG) to acrylamide beads to make immunobeads. The specificity and sensitivity of binding of human [125I]IgG to immunobeads permitted detection of as little as 10(-10) M unlabeled human IgG or aggregated human IgG (AHIgG) in competitive assay. The cells were incubated with an AHIgG concentration (3 x 10(-10) M) giving 25% inhibition in the RIA; unbound AHIgG concentration was then measured in the cell supernatants using RIA. Results show that Fc gamma receptors could be detected at 20 degrees C or at 37 degrees C (but not at 4 degrees C) on the four B cell lines tested. At whatever temperature of incubation, Fc gamma receptors were not detected on three T cell lines nor on two 'non T-non B' cell lines. This method allows screening of a large number of Fc gamma receptor positive cells. It is also useful for detecting Fc gamma receptors in membrane preparations or in detergent extracts of human B cell membranes. | lld:pubmed |
| pubmed-article:6969276 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6969276 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6969276 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6969276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6969276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6969276 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6969276 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:6969276 | pubmed:author | pubmed-author:FradeRR | lld:pubmed |
| pubmed-article:6969276 | pubmed:author | pubmed-author:BarelMM | lld:pubmed |
| pubmed-article:6969276 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6969276 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:6969276 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6969276 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6969276 | pubmed:pagination | 123-31 | lld:pubmed |
| pubmed-article:6969276 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:meshHeading | pubmed-meshheading:6969276-... | lld:pubmed |
| pubmed-article:6969276 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6969276 | pubmed:articleTitle | Detection of Fc gamma receptors on human lymphoblastoid cell surfaces using a simple solid phase radioimmunoassay specific for human IgG. | lld:pubmed |
| pubmed-article:6969276 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6969276 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
