| pubmed-article:6960240 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6960240 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
| pubmed-article:6960240 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:6960240 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:6960240 | lifeskim:mentions | umls-concept:C0008169 | lld:lifeskim |
| pubmed-article:6960240 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:6960240 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:6960240 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:6960240 | pubmed:dateCreated | 1983-2-14 | lld:pubmed |
| pubmed-article:6960240 | pubmed:abstractText | We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in this series, pSV2-cat, consisted of the beta-lactamase gene and origin of replication from pBR322 coupled to a simian virus 40 (SV40) early transcription region into which CAT coding sequences were inserted. Readily measured levels of CAT accumulated within 48 h after the introduction of pSV2-cat DNA into African green monkey kidney CV-1 cells. Because endogenous CAT activity is not present in CV-1 or other mammalian cells, and because rapid, sensitive assays for CAT activity are available, these recombinants provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells. To demonstrate the usefulness of this system, we constructed derivatives of pSV2-cat from which part or all of the SV40 promoter region was removed. Deletion of one copy of the 72-base-pair repeat sequence in the SV40 promoter caused no significant decrease in CAT synthesis in monkey kidney CV-1 cells; however, an additional deletion of 50 base pairs from the second copy of the repeats reduced CAT synthesis to 11% of its level in the wild type. We also constructed a recombinant, pSV0-cat, in which the entire SV40 promoter region was removed and a unique HindIII site was substituted for the insertion of other promoter sequences. | lld:pubmed |
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| pubmed-article:6960240 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6960240 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6960240 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6960240 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6960240 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:6960240 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:6960240 | pubmed:author | pubmed-author:HowardB HBH | lld:pubmed |
| pubmed-article:6960240 | pubmed:author | pubmed-author:GormanC MCM | lld:pubmed |
| pubmed-article:6960240 | pubmed:author | pubmed-author:MoffatL FLF | lld:pubmed |
| pubmed-article:6960240 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6960240 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:6960240 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6960240 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6960240 | pubmed:pagination | 1044-51 | lld:pubmed |
| pubmed-article:6960240 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
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| pubmed-article:6960240 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6960240 | pubmed:articleTitle | Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. | lld:pubmed |
| pubmed-article:6960240 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6960240 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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