| pubmed-article:6821514 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6821514 | lifeskim:mentions | umls-concept:C0015576 | lld:lifeskim |
| pubmed-article:6821514 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:6821514 | lifeskim:mentions | umls-concept:C0021873 | lld:lifeskim |
| pubmed-article:6821514 | lifeskim:mentions | umls-concept:C0600400 | lld:lifeskim |
| pubmed-article:6821514 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6821514 | pubmed:dateCreated | 1988-2-12 | lld:pubmed |
| pubmed-article:6821514 | pubmed:abstractText | The structural organization of intracisternal A-particle genes has been studied, using isolates from a mouse gene library in lambda phage Charon 4A. The predominant gene form among the isolates was 7.3 kilobases (kb) in length. R-loops between the 7-kb (35S) A-particle genomic ribonucleic acid and several of these genes were colinear, with no visible evidence of intervening deoxyribonucleic acid sequences. One recombinant was found with an A-particle gene that contained a 1.7-kb deletion. Using the deletion as a reference, the deoxyribonucleic acid and ribonucleic acid homology regions were localized with respect to one another and to the restriction map: the 5' terminus of the ribonucleic acid was several hundred base pairs within the 5' end of the deoxyribonucleic acid homology region. Restriction endonuclease fragments encompassing the 5' and 3' regions of one 7.3-kb gene were separately subcloned into pBR322. Heteroduplexes between the two subclones revealed an approximately 300-base pair segment of terminally redundant sequences. The cloned 3' fragment hybridized with restriction fragments from the 5' end of several other A-particle genes, demonstrating the presence of common (though not necessarily identical) terminally repeated sequences. A-particle genes varied in the occurrence of specific restriction sites at characteristic internal loci. However, heteroduplexes between several variant 7.3-kb genes showed continuous homology regions even when spread under stringent hybridization conditions. The relative abundance of restriction site variants was highly conserved in 12 laboratory strains of Mus musculus, in embryonic and adult tissues of a single inbred strain, and in the SC-1 cell line of feral mouse origin, but appeared to differ in a feral Japanese substrain, Mus musculus molossinus. Some evidence suggests that subsets of A-particle genes may have similar flanking sequences. The results are discussed in terms of the evolution of this multigene family. | lld:pubmed |
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| pubmed-article:6821514 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6821514 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6821514 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6821514 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6821514 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6821514 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6821514 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:6821514 | pubmed:author | pubmed-author:KuffE LEL | lld:pubmed |
| pubmed-article:6821514 | pubmed:author | pubmed-author:LuedersK KKK | lld:pubmed |
| pubmed-article:6821514 | pubmed:author | pubmed-author:SmithL ALA | lld:pubmed |
| pubmed-article:6821514 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6821514 | pubmed:volume | 1 | lld:pubmed |
| pubmed-article:6821514 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6821514 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6821514 | pubmed:pagination | 216-27 | lld:pubmed |
| pubmed-article:6821514 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:6821514 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6821514 | pubmed:articleTitle | Intracisternal A-particle genes in Mus musculus: a conserved family of retrovirus-like elements. | lld:pubmed |
| pubmed-article:6821514 | pubmed:affiliation | Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20205. | lld:pubmed |
| pubmed-article:6821514 | pubmed:publicationType | Journal Article | lld:pubmed |
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