| pubmed-article:6772444 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0027651 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0026764 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0021027 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C0439810 | lld:lifeskim |
| pubmed-article:6772444 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:6772444 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6772444 | pubmed:dateCreated | 1980-10-24 | lld:pubmed |
| pubmed-article:6772444 | pubmed:abstractText | A procedure is described for the large-scale purification of light (L) and heavy (H) chain mRNAs from plasmacytomas produced in mice. Intact RNA is selectively precipitated in high yield from frozen tumors homogenized in 3 M LiCl and 6 M urea. L and H-chain mRNAs were purified by oligo(dT)-cellulose chromatography and either sucrose gradient centrifugation in conditions preventing aggregation or by means of high-resolution preparative gel electrophoresis under non-denaturing conditions. gamma 2a and alpha H-chain mRNAs sedimented as major components at 15.5 S and 16.5 S respectively, when L-chain mRNAs sedimented as 12-S species. H-chain mRNAs isolated by continuous elution during preparative gel electrophoresis were completely separated from both L-chain mRNA and residual 18-S rRNA, and migrated as single components of 1900 +/- 50 nucleotides on analytical denaturing gels. The partially purified H-chain mRNAs were translated into major components of molecular weights of 56,000 (gamma 2a) and 60,000 (alpha) in an mRNA-dependent rabbit reticulocyte lysate, whereas L-chain mRNAs yielded polypeptides of molecular weights of 25,000 (gamma) and 27,000 (chi). Up to 95% of the translation products directed by the purified mRNAs were immunoprecipitated using specific antisera. The purity of L and H-chain mRNAs was assessed by hybridization of corresponding cDNAs with excess recombinant plasmid DNA. The results indicated a minimum purity of 47% (gamma 2a), 62% (alpha), for H-chain mRNAs and 60% (chi), for L-chain mRNAs. | lld:pubmed |
| pubmed-article:6772444 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6772444 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6772444 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6772444 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6772444 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:6772444 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:6772444 | pubmed:author | pubmed-author:RougeonFF | lld:pubmed |
| pubmed-article:6772444 | pubmed:author | pubmed-author:AuffrayCC | lld:pubmed |
| pubmed-article:6772444 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6772444 | pubmed:volume | 107 | lld:pubmed |
| pubmed-article:6772444 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6772444 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6772444 | pubmed:pagination | 303-14 | lld:pubmed |
| pubmed-article:6772444 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
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| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
| pubmed-article:6772444 | pubmed:meshHeading | pubmed-meshheading:6772444-... | lld:pubmed |
| pubmed-article:6772444 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6772444 | pubmed:articleTitle | Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. | lld:pubmed |
| pubmed-article:6772444 | pubmed:publicationType | Journal Article | lld:pubmed |
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