| pubmed-article:6749846 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C0036126 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C0023767 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C1301820 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:6749846 | lifeskim:mentions | umls-concept:C1518413 | lld:lifeskim |
| pubmed-article:6749846 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:6749846 | pubmed:dateCreated | 1982-12-2 | lld:pubmed |
| pubmed-article:6749846 | pubmed:abstractText | Endotoxin extracted from the heptose-less mutant of Salmonella typhimurium was hydrolyzed in 0.1 N HCl in methanol/water (1:1, v/v) at 100 degrees C to yield lipid A, which was then fractionated on a Sephadex LH-20 column to yield a major monophosphoryl lipid A fraction. The monophosphoryl lipid A was further fractionated by preparative thin layer chromatography. This process yielded three major bands (TLC-1, -3, and -5) and two minor bands (TLC-7 and -9). The purity of these fractions was established by ion exchange and reverse phase high performance liquid chromatography. The thin layer fractions were analyzed by fast atom bombardment mass spectrometry. TLC-1 and -3 gave molecular ions (M-H)- at m/e 1730 and 1716, respectively. Both of these fractions contained beta-hydroxymyristic, lauric, and 3-myristoxymyristic acids in O-acyl linkages. The molecular formula and Mr of TLC-1 are C95H179O22N2P and 1731.16; those of TLC-3 are C94H177O22N2P and 1717.15. TLC-1 was a methyl homolog of TLC-3. The major component of TLC-5 (C80H151O22N2P and Mr = 1506.99) gave a molecular ion at m/e 1506 and contained two beta-hydroxymyristic acids and a lauric acid in the O-acyl linkages. The major component of TLC-7 (C66H125O19N2P and Mr = 1280.83) and the single component of TLC-9 gave molecular ions at m/e 1280 and 1098, respectively. TLC-7 contained lauric and beta-hydroxymyristic acids in the O-acyl linkages. TLC-9 (C54H103O18N2P and Mr = 1098.69) contained a single O-acylated beta-hydroxymyristate group. TLC-1 and -3 were nontoxic in the chick embryo lethality test and regressed established tumors in the syngeneic guinea pigs. | lld:pubmed |
| pubmed-article:6749846 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6749846 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6749846 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6749846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6749846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6749846 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6749846 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:6749846 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:6749846 | pubmed:author | pubmed-author:TakayamaKK | lld:pubmed |
| pubmed-article:6749846 | pubmed:author | pubmed-author:QureshiNN | lld:pubmed |
| pubmed-article:6749846 | pubmed:author | pubmed-author:RibiEE | lld:pubmed |
| pubmed-article:6749846 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6749846 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:6749846 | pubmed:volume | 257 | lld:pubmed |
| pubmed-article:6749846 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6749846 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6749846 | pubmed:pagination | 11808-15 | lld:pubmed |
| pubmed-article:6749846 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:6749846 | pubmed:meshHeading | pubmed-meshheading:6749846-... | lld:pubmed |
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| pubmed-article:6749846 | pubmed:meshHeading | pubmed-meshheading:6749846-... | lld:pubmed |
| pubmed-article:6749846 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6749846 | pubmed:articleTitle | Purification and structural determination of nontoxic lipid A obtained from the lipopolysaccharide of Salmonella typhimurium. | lld:pubmed |
| pubmed-article:6749846 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6749846 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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