| Subject | Predicate | Object | Context |
|---|---|---|---|
| pubmed-article:6743650 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6743650 | lifeskim:mentions | umls-concept:C0030411 | lld:lifeskim |
| pubmed-article:6743650 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:6743650 | lifeskim:mentions | umls-concept:C0220926 | lld:lifeskim |
| pubmed-article:6743650 | lifeskim:mentions | umls-concept:C1159576 | lld:lifeskim |
| pubmed-article:6743650 | lifeskim:mentions | umls-concept:C0972349 | lld:lifeskim |
| pubmed-article:6743650 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6743650 | pubmed:dateCreated | 1984-9-12 | lld:pubmed |
| pubmed-article:6743650 | pubmed:abstractText | A fast-responding O2 electrode has been used to confirm and extend observations of a significant kinetic discrepancy between O2 reduction and consequent proton translocation in 'O2-pulse' experiments in intact cells of P. denitrificans. The permeant, chaotropic SCN- ion abolishes this discrepancy, and greatly increases the observable----H+/O ratio, to a value approaching its accepted, true, limiting stoichiometry. The observable H+ decay rates are very slow, particularly in the absence of SCN-. The submaximal----H+/O ratios observed in the absence of SCN- are essentially independent of the size of the O2 pulse, in a manner not easily explained by a delocalised chemiosmotic energy-coupling scheme. Osmotically active protoplasts of P. denitrificans do not show a significant kinetic discrepancy between O2 reduction and H+ translocation, even in the the absence of SCN-. However, the submaximal----H+/O ratios observed in the absence of SCN- are again essentially independent of the size of the O2 pulse. As in intact cells, the observable H+ decay rates are very slow. The energy-transfer inhibitor venturicidin causes a significant increase in the----H+/O ratio observed in protoplasts of P. denitrificans in the absence of SCN-; the decay kinetics of the H+ translocation process are also somewhat modified. Nevertheless, the----H+/O ratio observed in the presence of venturicidin is also independent of the size of the O2 pulse. This observation militates further against arguments in which (a) a non-ohmic leak of protons from the bulk aqueous phase might alone be the cause of the low----H+/O ratios observed in the absence of SCN-, and (b) in which there might be a delta p-dependent change ('redox slip') in the actual----H+/O ratio. It is concluded that the observable protonmotive activity of the respiratory chain of P. denitrificans in the absence of SCN- is directly influenced by the state of the H+-ATP synthetase in the cytoplasmic membrane of this organism. We are unable to explain the data in terms of a model in which the putative protonmotive force may be acting to affect the----H+/O ratio. The possibility is considered that the delocalised bulk-to-bulk phase membrane potential set up in response to protonmotive activity is energetically insignificant. | lld:pubmed |
| pubmed-article:6743650 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6743650 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6743650 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6743650 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6743650 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6743650 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6743650 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6743650 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:6743650 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:6743650 | pubmed:author | pubmed-author:KellD BDB | lld:pubmed |
| pubmed-article:6743650 | pubmed:author | pubmed-author:HitchensG DGD | lld:pubmed |
| pubmed-article:6743650 | pubmed:issnType | lld:pubmed | |
| pubmed-article:6743650 | pubmed:day | 27 | lld:pubmed |
| pubmed-article:6743650 | pubmed:volume | 766 | lld:pubmed |
| pubmed-article:6743650 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6743650 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6743650 | pubmed:pagination | 222-32 | lld:pubmed |
| pubmed-article:6743650 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:meshHeading | pubmed-meshheading:6743650-... | lld:pubmed |
| pubmed-article:6743650 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6743650 | pubmed:articleTitle | On the effects of thiocyanate and venturicidin on respiration-driven proton translocation in Paracoccus denitrificans. | lld:pubmed |
| pubmed-article:6743650 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6743650 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6743650 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6743650 | lld:pubmed |