| pubmed-article:6614909 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6614909 | lifeskim:mentions | umls-concept:C0004663 | lld:lifeskim |
| pubmed-article:6614909 | lifeskim:mentions | umls-concept:C0027803 | lld:lifeskim |
| pubmed-article:6614909 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6614909 | pubmed:dateCreated | 1983-10-21 | lld:pubmed |
| pubmed-article:6614909 | pubmed:abstractText | A neuraminidase from Bacteroides fragilis was purified 542-fold by isoelectric focusing, adsorption chromatography on Affi-Gel 202, and gel filtration chromatography on Sephadex G-200. On isoelectric focusing the neuraminidase was resolved into three differently charged fractions with pI values of 6.8, 7.1, and 7.4. The major component of pI 7.1 was used for further purification. The purified enzyme had optimal activity at pH 6.4 with N-acetylneuraminlactose as the substrate. Its molecular weight, determined by Sephadex G-200 gel filtration chromatography, was 92,000. The neuraminidase hydrolyzed terminal neuraminic acid residues from N-acetylneuraminlactose, fetuin, bovine submaxillary mucin, and porcine stomach lining mucin. A new method for the detection of neuraminidase activity is described which is based on rocket affinoelectrophoresis. It utilizes the differences in the interaction of sialylated and desialylated mucin with Helix pomatia lectin, enzymatic activity being detected by formation of affinorockets after incubation of the neuraminidase with bovine submaxillary mucin. | lld:pubmed |
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| pubmed-article:6614909 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6614909 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6614909 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6614909 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6614909 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:6614909 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:6614909 | pubmed:author | pubmed-author:NordC ECE | lld:pubmed |
| pubmed-article:6614909 | pubmed:author | pubmed-author:BergJ OJO | lld:pubmed |
| pubmed-article:6614909 | pubmed:author | pubmed-author:AnderssonGG | lld:pubmed |
| pubmed-article:6614909 | pubmed:author | pubmed-author:LindqvistLL | lld:pubmed |
| pubmed-article:6614909 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6614909 | pubmed:volume | 46 | lld:pubmed |
| pubmed-article:6614909 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6614909 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6614909 | pubmed:pagination | 75-80 | lld:pubmed |
| pubmed-article:6614909 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6614909 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6614909 | pubmed:articleTitle | Neuraminidase in Bacteroides fragilis. | lld:pubmed |
| pubmed-article:6614909 | pubmed:publicationType | Journal Article | lld:pubmed |
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