| pubmed-article:6606613 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6606613 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:6606613 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:6606613 | lifeskim:mentions | umls-concept:C0439158 | lld:lifeskim |
| pubmed-article:6606613 | lifeskim:mentions | umls-concept:C0039654 | lld:lifeskim |
| pubmed-article:6606613 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:6606613 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:6606613 | pubmed:dateCreated | 1984-2-14 | lld:pubmed |
| pubmed-article:6606613 | pubmed:abstractText | Colony growth of human lymphocytes by phorbol myristate acetate (PMA) was studied. PMA was able to induce lymphocyte colony growth in methylcellulose semi-solid cultures in the absence of lectin mitogens, phytohaemagglutinin or concanavalin A. PMA-induced colonies were found in cultures of mononuclear cells, monocyte-depleted mononuclear cells and the T-enriched cell fraction, whereas no colonies were obtained from the non-T cell fraction. At least one million mononuclear cells were required to form colonies by PMA. The colony cells were mainly T cells as judged from sheep red blood cell rosette formation. Surface immunoglobulin positive cells and peroxidase positive cells were not detected in colony cells. Non-specific esterase positive cells were only found to be less than 1% of colony cells. T cells formed more colonies than did mononuclear cells, presumably because of a concentration of colony forming cells and/or co-operating cells. Colony formation by PMA was induced from monocyte-depleted mononuclear cells and monocyte-depleted T cells, suggesting independence of monocytes. When mononuclear cells were precultured at 37 degrees for 5 min or 30 min with PMA and then cultured in the semi-solid medium without PMA, only a small number of colonies grew. Further studies of PMA-induced T-cell colonies will provide information the identification and characterization of immunological states in various immunological diseases. | lld:pubmed |
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| pubmed-article:6606613 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6606613 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6606613 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6606613 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6606613 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6606613 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6606613 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:6606613 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:6606613 | pubmed:author | pubmed-author:KishiTT | lld:pubmed |
| pubmed-article:6606613 | pubmed:author | pubmed-author:WESSA GAG | lld:pubmed |
| pubmed-article:6606613 | pubmed:author | pubmed-author:SakanoTT | lld:pubmed |
| pubmed-article:6606613 | pubmed:author | pubmed-author:HyodoSS | lld:pubmed |
| pubmed-article:6606613 | pubmed:author | pubmed-author:SuzawaTT | lld:pubmed |
| pubmed-article:6606613 | pubmed:author | pubmed-author:KashiwaHH | lld:pubmed |
| pubmed-article:6606613 | pubmed:author | pubmed-author:KittakaEE | lld:pubmed |
| pubmed-article:6606613 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6606613 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:6606613 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6606613 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6606613 | pubmed:pagination | 575-80 | lld:pubmed |
| pubmed-article:6606613 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6606613 | pubmed:meshHeading | pubmed-meshheading:6606613-... | lld:pubmed |
| pubmed-article:6606613 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6606613 | pubmed:articleTitle | Induction of human T-lymphocyte colonies by phorbol myristate acetate. | lld:pubmed |
| pubmed-article:6606613 | pubmed:publicationType | Journal Article | lld:pubmed |
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