| pubmed-article:6578502 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6578502 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:6578502 | lifeskim:mentions | umls-concept:C0062108 | lld:lifeskim |
| pubmed-article:6578502 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:6578502 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:6578502 | pubmed:dateCreated | 1983-11-23 | lld:pubmed |
| pubmed-article:6578502 | pubmed:abstractText | We have developed a procedure for the purification of halorhodopsin in a photochemically active state. Solubilization of membranes from a bacteriorhodopsin-negative Halobacterium strain with octyl glucoside was followed by chromatography on hydroxylapatite and octyl-Sepharose gels. All steps were carried out in high-ionic-strength solutions. The procedure resulted in 270-fold enrichment with a 35% yield. The eluted pigment had an absorption maximum at 575 nm and an A280/A575 ratio of 2. On removal of the detergent by dialysis, the purified halorhodopsin was chemically bleached, regenerated with [3H]retinal, and reduced with cyanoborohydride. Such samples showed one main and one satellite band after staining or fluorography of NaDodSO4/polyacrylamide gels. The apparent molecular weight of the main band was 25,000. Purified halorhodopsin underwent a photocycle after excitation with pulsed laser light and showed a 9-nm blue shift (at neutral pH) on removal of chloride ion. The pigment also underwent a photoreversible shift at alkaline pH to a form absorbing maximally at 410 nm. All three reactions closely resembled those of membrane-bound halorhodopsin. | lld:pubmed |
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| pubmed-article:6578502 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6578502 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6578502 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6578502 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:6578502 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6578502 | pubmed:author | pubmed-author:BogomolniR... | lld:pubmed |
| pubmed-article:6578502 | pubmed:author | pubmed-author:WeberH JHJ | lld:pubmed |
| pubmed-article:6578502 | pubmed:author | pubmed-author:TaylorM EME | lld:pubmed |
| pubmed-article:6578502 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6578502 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:6578502 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6578502 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6578502 | pubmed:pagination | 6172-6 | lld:pubmed |
| pubmed-article:6578502 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
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| pubmed-article:6578502 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6578502 | pubmed:articleTitle | Purification of photochemically active halorhodopsin. | lld:pubmed |
| pubmed-article:6578502 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6578502 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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