| pubmed-article:6476376 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6476376 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:6476376 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:6476376 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:6476376 | lifeskim:mentions | umls-concept:C1553879 | lld:lifeskim |
| pubmed-article:6476376 | lifeskim:mentions | umls-concept:C0076413 | lld:lifeskim |
| pubmed-article:6476376 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6476376 | pubmed:dateCreated | 1984-9-26 | lld:pubmed |
| pubmed-article:6476376 | pubmed:abstractText | A rapid and simple method for assaying the binding activity of thiamine-binding protein is described. By this assay method, the binding characteristics of rice bran thiamine-binding protein have been evaluated with [14C]thiamine as ligand. Analysis of these data by Scatchard plot resulted in linear plots giving a dissociation constant (Kd) for thiamine of 0.55 microM and a maximum binding (Bmax) of 14.5 pmol of ligand bound/microgram of protein. Thiamine binding to the binding protein was time dependent and reached equilibrium at approximately 20 min. The Kob was 0.18 min-1 and the k1 was 1.25 X 10(5) min-1 M-1. Reversibility of thiamine binding at equilibrium was completed at 60 min with a k2 value of 0.052 min-1. The Kd calculated from the reverse rate constant was 0.42 microM. These results indicated that this binding assay method was substantially reliable and accurate. | lld:pubmed |
| pubmed-article:6476376 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6476376 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6476376 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6476376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6476376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6476376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6476376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6476376 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6476376 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:6476376 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:6476376 | pubmed:author | pubmed-author:YoshiokaKK | lld:pubmed |
| pubmed-article:6476376 | pubmed:author | pubmed-author:NishimuraHH | lld:pubmed |
| pubmed-article:6476376 | pubmed:author | pubmed-author:IwashimaAA | lld:pubmed |
| pubmed-article:6476376 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6476376 | pubmed:volume | 139 | lld:pubmed |
| pubmed-article:6476376 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6476376 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6476376 | pubmed:pagination | 373-6 | lld:pubmed |
| pubmed-article:6476376 | pubmed:dateRevised | 2002-11-1 | lld:pubmed |
| pubmed-article:6476376 | pubmed:meshHeading | pubmed-meshheading:6476376-... | lld:pubmed |
| pubmed-article:6476376 | pubmed:meshHeading | pubmed-meshheading:6476376-... | lld:pubmed |
| pubmed-article:6476376 | pubmed:meshHeading | pubmed-meshheading:6476376-... | lld:pubmed |
| pubmed-article:6476376 | pubmed:meshHeading | pubmed-meshheading:6476376-... | lld:pubmed |
| pubmed-article:6476376 | pubmed:meshHeading | pubmed-meshheading:6476376-... | lld:pubmed |
| pubmed-article:6476376 | pubmed:meshHeading | pubmed-meshheading:6476376-... | lld:pubmed |
| pubmed-article:6476376 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6476376 | pubmed:articleTitle | A method for determining binding kinetics applied to thiamine-binding protein. | lld:pubmed |
| pubmed-article:6476376 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6476376 | lld:pubmed |
