| pubmed-article:6459780 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C2752508 | lld:lifeskim |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C0036751 | lld:lifeskim |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C1325604 | lld:lifeskim |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C1167322 | lld:lifeskim |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C0679823 | lld:lifeskim |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C0812409 | lld:lifeskim |
| pubmed-article:6459780 | lifeskim:mentions | umls-concept:C0033727 | lld:lifeskim |
| pubmed-article:6459780 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6459780 | pubmed:dateCreated | 1982-3-13 | lld:pubmed |
| pubmed-article:6459780 | pubmed:abstractText | Dense granules, the storage organelles for 5-hydroxytryptamine in blood platelets, have been isolated from porcine platelets and are shown to transport 5-hydroxytryptamine in response to a transmembrane proton gradient (delta pH). Transport in the absence of delta pH is minimal, and it is shown that a rapid increase in transport takes place as delta pH increases. Direct measurements with [14C]methylamine show a delta pH of 1.1 units (acid inside) for intact granules. Osmotically active ghosts of dense granules from which 95% of the endogenous 5-hydroxytryptamine content has been released have also been prepared. Ghosts swell in the presence of ATP and Mg2+, and this swelling is shown to be due to the entry of protons via a process linked to ATP hydrolysis. Proton entry is also apparently linked to anion penetration in ghosts. Steady-state 5-hydroxytryptamine transport in ghosts is stimulated approx. 3-fold on the addition of ATP to the incubation medium, and the stimulation of 5-hydroxytryptamine transport in ghosts correlates with the formation of a transmembrane delta pH. Ghosts generate a delta pH of 1.1-1.3 pH units (acid inside) in the presence of 5 mM-ATP/2.5 mM-MgSO4. delta pH is generated within 3 min at 37 degrees C and is dissipated by the ionophore nigericin and by NH4Cl. It is shown that an Mg2+-stimulated ATPase activity is present on the ghost membrane, and inhibition of the ATPase leads to a corresponding decrease in 5-hydroxytryptamine transport. The results presented support the idea that 5-hydroxytryptamine transport into platelet dense granules is dependent on the presence of a transmembrane delta pH and, together with previous findings by others, suggest a generalized mechanism for biogenic amine transport into subcellular storage organelles. | lld:pubmed |
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| pubmed-article:6459780 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6459780 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6459780 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6459780 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6459780 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:6459780 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:6459780 | pubmed:author | pubmed-author:SalganicoffLL | lld:pubmed |
| pubmed-article:6459780 | pubmed:author | pubmed-author:WilkinsJ AJA | lld:pubmed |
| pubmed-article:6459780 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6459780 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:6459780 | pubmed:volume | 198 | lld:pubmed |
| pubmed-article:6459780 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6459780 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6459780 | pubmed:pagination | 113-23 | lld:pubmed |
| pubmed-article:6459780 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6459780 | pubmed:meshHeading | pubmed-meshheading:6459780-... | lld:pubmed |
| pubmed-article:6459780 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6459780 | pubmed:articleTitle | Participation of a transmembrane proton gradient in 5-hydroxytryptamine transport by platelet dense granules and dense-granule ghosts. | lld:pubmed |
| pubmed-article:6459780 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6459780 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:6459780 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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