pubmed-article:6442732 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:6442732 | lifeskim:mentions | umls-concept:C0002430 | lld:lifeskim |
pubmed-article:6442732 | lifeskim:mentions | umls-concept:C0018787 | lld:lifeskim |
pubmed-article:6442732 | lifeskim:mentions | umls-concept:C0077404 | lld:lifeskim |
pubmed-article:6442732 | lifeskim:mentions | umls-concept:C0475264 | lld:lifeskim |
pubmed-article:6442732 | pubmed:dateCreated | 1985-6-13 | lld:pubmed |
pubmed-article:6442732 | pubmed:abstractText | Troponin-T (Tn-T) expression in developing hearts of axolotls, Ambystoma mexicanum, was studied with the use of polyclonal and monoclonal antibodies and SDS-polyacrylamide gel electrophoresis. In precontractile hearts (stage 32/33), Tn-T was present in addition to myosin, actin and tropomyosin as evidenced by the presence of the protein bands in SDS-gels and by indirect immunofluorescence. Tn-T was localized in amorphous collections at the peripheries of these precontractile cells. Hearts of normal and cardiac lethal mutant siblings were also analysed for Tn-T expression. No detectable differences in the quantity of protein present was observed by gel electrophoresis or by indirect immuno-fluorescence. The most striking difference concerned the localization of the protein. In normal hearts, Tn-T was primarily localized in the I-bands of organized myofibrils; however, in mutant cells the Tn-T was localized in amorphous collections at the cell peripheries suggesting a reduction of myofibrillar organization in these cells. No differences were observed in the contractile protein composition between normal and mutant embryonic hearts by gel electrophoresis experiments. | lld:pubmed |
pubmed-article:6442732 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:language | eng | lld:pubmed |
pubmed-article:6442732 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:6442732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6442732 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:6442732 | pubmed:month | Dec | lld:pubmed |
pubmed-article:6442732 | pubmed:issn | 0022-0752 | lld:pubmed |
pubmed-article:6442732 | pubmed:author | pubmed-author:GreaserM LML | lld:pubmed |
pubmed-article:6442732 | pubmed:author | pubmed-author:LemanskiL FLF | lld:pubmed |
pubmed-article:6442732 | pubmed:author | pubmed-author:PAZM RMR | lld:pubmed |
pubmed-article:6442732 | pubmed:author | pubmed-author:FuldnerR ARA | lld:pubmed |
pubmed-article:6442732 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:6442732 | pubmed:volume | 84 | lld:pubmed |
pubmed-article:6442732 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:6442732 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:6442732 | pubmed:pagination | 1-17 | lld:pubmed |
pubmed-article:6442732 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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pubmed-article:6442732 | pubmed:year | 1984 | lld:pubmed |
pubmed-article:6442732 | pubmed:articleTitle | Accumulation and localization of troponin-T in developing hearts of Ambystoma mexicanum. | lld:pubmed |
pubmed-article:6442732 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:6442732 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:6442732 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |