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pubmed-article:6442732pubmed:abstractTextTroponin-T (Tn-T) expression in developing hearts of axolotls, Ambystoma mexicanum, was studied with the use of polyclonal and monoclonal antibodies and SDS-polyacrylamide gel electrophoresis. In precontractile hearts (stage 32/33), Tn-T was present in addition to myosin, actin and tropomyosin as evidenced by the presence of the protein bands in SDS-gels and by indirect immunofluorescence. Tn-T was localized in amorphous collections at the peripheries of these precontractile cells. Hearts of normal and cardiac lethal mutant siblings were also analysed for Tn-T expression. No detectable differences in the quantity of protein present was observed by gel electrophoresis or by indirect immuno-fluorescence. The most striking difference concerned the localization of the protein. In normal hearts, Tn-T was primarily localized in the I-bands of organized myofibrils; however, in mutant cells the Tn-T was localized in amorphous collections at the cell peripheries suggesting a reduction of myofibrillar organization in these cells. No differences were observed in the contractile protein composition between normal and mutant embryonic hearts by gel electrophoresis experiments.lld:pubmed
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pubmed-article:6442732pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:6442732pubmed:year1984lld:pubmed
pubmed-article:6442732pubmed:articleTitleAccumulation and localization of troponin-T in developing hearts of Ambystoma mexicanum.lld:pubmed
pubmed-article:6442732pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:6442732pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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