| pubmed-article:6433901 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C0005821 | lld:lifeskim |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C0021868 | lld:lifeskim |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C0013855 | lld:lifeskim |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C1522240 | lld:lifeskim |
| pubmed-article:6433901 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:6433901 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6433901 | pubmed:dateCreated | 1984-10-24 | lld:pubmed |
| pubmed-article:6433901 | pubmed:abstractText | By using density-gradient fractionation and high-voltage free-flow electrophoresis, human platelet membranes were separated into highly purified subfractions of surface (SM) and intracellular (IM) origin. Associated exclusively with the IM fraction is an ATP-dependent Ca2+ uptake that, in the absence of oxalate, reaches steady-state levels in 5-10 min. When Ca2+-EGTA buffers were used to control the external Ca2+ concentrations (range 0.1-50 microM) there was an increase in the intravesicle steady-state level of Ca2+ up to 10 microM external Ca2+ concentration. Above this level the intravesicle space becomes saturated at a concentration between 10 and 20 nmol of Ca2+ X (mg of protein)-1. The ionophore A23187 promotes a rapid and almost total release of the sequestered Ca2+ (greater than 90%, t1/2 1-2 min). The presence of oxalate in the external medium greatly enhances the Ca2+ accumulation to levels as high as 200 nmol X (mg of protein)-1, but the uptake process is more variable and rarely reaches steady-state level even after 2 h incubation. Moreover, accumulation in the presence of oxalate effects ionophore release with less than 80% depletion in 45-60 min. These findings, taken together with the known presence in the platelet of a wide variety of functional and metabolic processes triggered by this cation, suggest that the platelet IM has a key role in controlling cytosolic Ca2+ concentrations. | lld:pubmed |
| pubmed-article:6433901 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6433901 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6433901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6433901 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6433901 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:6433901 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:6433901 | pubmed:author | pubmed-author:CrawfordNN | lld:pubmed |
| pubmed-article:6433901 | pubmed:author | pubmed-author:MenashiSS | lld:pubmed |
| pubmed-article:6433901 | pubmed:author | pubmed-author:AuthiK SKS | lld:pubmed |
| pubmed-article:6433901 | pubmed:author | pubmed-author:CareyFF | lld:pubmed |
| pubmed-article:6433901 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6433901 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:6433901 | pubmed:volume | 222 | lld:pubmed |
| pubmed-article:6433901 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6433901 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6433901 | pubmed:pagination | 413-7 | lld:pubmed |
| pubmed-article:6433901 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:meshHeading | pubmed-meshheading:6433901-... | lld:pubmed |
| pubmed-article:6433901 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6433901 | pubmed:articleTitle | Characterization of the calcium-sequestering process associated with human platelet intracellular membranes isolated by free-flow electrophoresis. | lld:pubmed |
| pubmed-article:6433901 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6433901 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6433901 | lld:pubmed |
