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pubmed-article:6366058pubmed:abstractTextA method is described for the preparation of human peripheral blood mononuclear cell (PBMC) suspensions containing highly enriched natural killer (NK) cell cytotoxicity. The technique involved the negative selection of OKM1+ cells by the selective removal of nylon wool nonadherent PBMC which are reactive with the Leu-1 monoclonal antibody. The Leu-1+ cells are removed by subsequent rosette formation with anti-mouse IgG coated bovine erythrocytes. The resultant OKM1+ cell suspension had a greater number of large granular lymphocytes, K562 target binding effector cells, and lytic activity than concomitantly prepared fractions of Percoll gradients.lld:pubmed
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pubmed-article:6366058pubmed:articleTitleEnrichment of natural killer cells by negative selection: comparison to Percoll gradient separation method.lld:pubmed
pubmed-article:6366058pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:6366058pubmed:publicationTypeComparative Studylld:pubmed
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