| pubmed-article:6353412 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0521447 | lld:lifeskim |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0521451 | lld:lifeskim |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0030933 | lld:lifeskim |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0009219 | lld:lifeskim |
| pubmed-article:6353412 | lifeskim:mentions | umls-concept:C0917791 | lld:lifeskim |
| pubmed-article:6353412 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:6353412 | pubmed:dateCreated | 1983-11-23 | lld:pubmed |
| pubmed-article:6353412 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6353412 | pubmed:abstractText | A 3.1-kilobase Bgl II fragment of Saccharomyces cerevisiae carrying the nuclear gene encoding the mitochondrial polypeptide chain elongation factor (EF) Tu has been cloned on pBR327 to yield a chimeric plasmid pYYB. The identification of the gene designated as tufM was based on the cross-hybridization with the Escherichia coli tufB gene, under low stringency conditions. The complete nucleotide sequence of the yeast tufM gene was established together with its 5'- and 3'-flanking regions. The sequence contained 1,311 nucleotides coding for a protein of 437 amino acids with a calculated Mr of 47,980. The nucleotide sequence and the deduced amino acid sequence of tufM were 60% and 66% homologous, respectively, to the corresponding sequences of E. coli tufA, when aligned to obtain the maximal homology. Plasmid YRpYB was then constructed by cloning the 2.5-kilobase EcoRI fragment of pYYB carrying tufM into a yeast cloning vector YRp-7. A mRNA hybridizable with tufM was isolated from the total mRNA of S. cerevisiae D13-1A transformed with YRpYB and translated in the reticulocyte lysate. The mRNA could direct the synthesis of a protein with Mr 48,000, which was immunoprecipitated with an anti-E. coli EF-Tu antibody but not with an antibody against yeast cytoplasmic EF-1 alpha. The results indicate that the tufM gene is a nuclear gene coding for the yeast mitochondrial EF-Tu. | lld:pubmed |
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| pubmed-article:6353412 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6353412 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6353412 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6353412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6353412 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6353412 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:6353412 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6353412 | pubmed:author | pubmed-author:NaitoAA | lld:pubmed |
| pubmed-article:6353412 | pubmed:author | pubmed-author:NagataSS | lld:pubmed |
| pubmed-article:6353412 | pubmed:author | pubmed-author:KaziroYY | lld:pubmed |
| pubmed-article:6353412 | pubmed:author | pubmed-author:Tsunetsugu-Yo... | lld:pubmed |
| pubmed-article:6353412 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6353412 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:6353412 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6353412 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6353412 | pubmed:pagination | 6192-6 | lld:pubmed |
| pubmed-article:6353412 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:6353412 | pubmed:meshHeading | pubmed-meshheading:6353412-... | lld:pubmed |
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| pubmed-article:6353412 | pubmed:meshHeading | pubmed-meshheading:6353412-... | lld:pubmed |
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| pubmed-article:6353412 | pubmed:meshHeading | pubmed-meshheading:6353412-... | lld:pubmed |
| pubmed-article:6353412 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6353412 | pubmed:articleTitle | Molecular cloning and sequence determination of the nuclear gene coding for mitochondrial elongation factor Tu of Saccharomyces cerevisiae. | lld:pubmed |
| pubmed-article:6353412 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:854359 | entrezgene:pubmed | pubmed-article:6353412 | lld:entrezgene |
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