| pubmed-article:6346945 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6346945 | lifeskim:mentions | umls-concept:C0018353 | lld:lifeskim |
| pubmed-article:6346945 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:6346945 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:6346945 | lifeskim:mentions | umls-concept:C0008551 | lld:lifeskim |
| pubmed-article:6346945 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6346945 | pubmed:dateCreated | 1983-8-11 | lld:pubmed |
| pubmed-article:6346945 | pubmed:abstractText | A method for the synthesis and purification of guanosine 5'-[gamma-S]triphosphate labeled with 32P in the beta-position is described. The first step in the synthesis involves the quantitative transfer of 32Pi from [gamma-32P]dATP to 5'-GMP catalyzed by GMP kinase. Further incubation of the beta-32P]GDP product with [gamma-S]GTP and nucleoside diphosphate kinase results in the synthesis of [beta-32P][gamma-S]GTP with a yield of 10 to 18%. The 32P-labeled [gamma-S]nucleotide is purified by binding to mercury-agarose and eluting with buffer containing beta-mercaptoethanol. Specific incorporation of 32P into the beta-position was demonstrated by treating [beta-32P][gamma-S]GTP with 7% formic acid to remove the gamma-thiophosphate and digesting the remaining [beta-32P]GDP with nucleotide pyro-phosphatase. Although 5'-GMP was released after pyrophosphatase digestion, the only 32P radioactivity detected was as inorganic phosphate. | lld:pubmed |
| pubmed-article:6346945 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6346945 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6346945 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6346945 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6346945 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6346945 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6346945 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6346945 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6346945 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6346945 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6346945 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:6346945 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:6346945 | pubmed:author | pubmed-author:HuangR CRC | lld:pubmed |
| pubmed-article:6346945 | pubmed:author | pubmed-author:ReeveA EAE | lld:pubmed |
| pubmed-article:6346945 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6346945 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:6346945 | pubmed:volume | 130 | lld:pubmed |
| pubmed-article:6346945 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6346945 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6346945 | pubmed:pagination | 14-8 | lld:pubmed |
| pubmed-article:6346945 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:6346945 | pubmed:meshHeading | pubmed-meshheading:6346945-... | lld:pubmed |
| pubmed-article:6346945 | pubmed:meshHeading | pubmed-meshheading:6346945-... | lld:pubmed |
| pubmed-article:6346945 | pubmed:meshHeading | pubmed-meshheading:6346945-... | lld:pubmed |
| pubmed-article:6346945 | pubmed:meshHeading | pubmed-meshheading:6346945-... | lld:pubmed |
| pubmed-article:6346945 | pubmed:meshHeading | pubmed-meshheading:6346945-... | lld:pubmed |
| pubmed-article:6346945 | pubmed:meshHeading | pubmed-meshheading:6346945-... | lld:pubmed |
| pubmed-article:6346945 | pubmed:meshHeading | pubmed-meshheading:6346945-... | lld:pubmed |
| pubmed-article:6346945 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6346945 | pubmed:articleTitle | Synthesis and affinity purification of beta-32P-labeled [gamma-S]GTP. | lld:pubmed |
| pubmed-article:6346945 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6346945 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
