| pubmed-article:6339817 | pubmed:abstractText | The free epigastric rabbit flap was used to observe morphological alterations in the microvasculature following prolonged hypothermic ischemic intervals. Free flaps were raised, cooled 1-6 days, and reanastomosed. The epigastric artery, vein, and dermal vessels were examined by electron microscopy. Efforts to keep flap tissues in the cold less than or equal to 5 days were successful (85% of flaps survived following reanastomosis N = 15). Flap failures were accompanied by extensive disruption to the endothelial cells of the epigastric vein. The findings support the efficacy of a cooling period less than or equal to 5 days for free flaps. The morphological alterations in the venous endothelium of the flap also suggest a plausible explanation for flap failures which occur in spite of patent anastomotic sites. | lld:pubmed |
