| pubmed-article:6337848 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6337848 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:6337848 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:6337848 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:6337848 | lifeskim:mentions | umls-concept:C0178655 | lld:lifeskim |
| pubmed-article:6337848 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6337848 | pubmed:dateCreated | 1983-4-7 | lld:pubmed |
| pubmed-article:6337848 | pubmed:abstractText | In this paper we have studied the effect of raising the intracellular EF-Tu concentration on the expression of tufB. To this aim cells were transformed with multicopy plasmids carrying either tufA or tufB. The intracellular EF-Tu concentrations were determined by the specific immunoelectrophoresis assay described in the preceding paper in this journal. We have cloned the tufA gene in a plasmid, containing the powerful major leftward promoter (PL) of phage lambda. Transcription from PL can be repressed at low temperature by a temperature-sensitive repressor and activated by heat induction. Cloning occurred in two orientations in a single EcoRI site about 150 base pairs downstream of PL. Cells carrying either plasmid were shown to contain an almost doubled amount of EF-Tu at temperatures from 28 degrees C to 37 degrees C. This indicates that transcription of tufA can proceed from a possible binding site for RNA polymerase on these cloned fragments. The EF-Tu level was further increased to about 30% of total cellular protein after a temperature shift from 37 degrees C to 43 degrees C. The multicopy plasmid pTuB1 described by Miyajima et al. [FEBS Lett. 102, 207-210 (1979)] and a derivative (pTuBo, compare preceding paper in this journal) were used to study the expression of both chromosomal and plasmid-borne tufB. Transformation with either plasmid raised the intracellular EF-Tu concentration by 30-60% depending on the nutritional conditions. Suppression of tufB expression was observed when the intracellular level of EF-Tu increased after transformation with all plasmids mentioned above. The results are in accord with the concept that EF-Tu acts as an autogenous feedback inhibitor involved in the regulation of tufB. | lld:pubmed |
| pubmed-article:6337848 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6337848 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6337848 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6337848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6337848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6337848 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6337848 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:6337848 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:6337848 | pubmed:author | pubmed-author:BoschLL | lld:pubmed |
| pubmed-article:6337848 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:6337848 | pubmed:author | pubmed-author:KasteleinR... | lld:pubmed |
| pubmed-article:6337848 | pubmed:author | pubmed-author:VijgenboomEE | lld:pubmed |
| pubmed-article:6337848 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6337848 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:6337848 | pubmed:volume | 130 | lld:pubmed |
| pubmed-article:6337848 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6337848 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6337848 | pubmed:pagination | 409-17 | lld:pubmed |
| pubmed-article:6337848 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:6337848 | pubmed:meshHeading | pubmed-meshheading:6337848-... | lld:pubmed |
| pubmed-article:6337848 | pubmed:meshHeading | pubmed-meshheading:6337848-... | lld:pubmed |
| pubmed-article:6337848 | pubmed:meshHeading | pubmed-meshheading:6337848-... | lld:pubmed |
| pubmed-article:6337848 | pubmed:meshHeading | pubmed-meshheading:6337848-... | lld:pubmed |
| pubmed-article:6337848 | pubmed:meshHeading | pubmed-meshheading:6337848-... | lld:pubmed |
| pubmed-article:6337848 | pubmed:meshHeading | pubmed-meshheading:6337848-... | lld:pubmed |
| pubmed-article:6337848 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6337848 | pubmed:articleTitle | tuf gene dosage effects on the intracellular concentration of EF-TuB. | lld:pubmed |
| pubmed-article:6337848 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6337848 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6337848 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6337848 | lld:pubmed |
