| pubmed-article:6329916 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6329916 | lifeskim:mentions | umls-concept:C0446021 | lld:lifeskim |
| pubmed-article:6329916 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:6329916 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:6329916 | lifeskim:mentions | umls-concept:C0022487 | lld:lifeskim |
| pubmed-article:6329916 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:6329916 | lifeskim:mentions | umls-concept:C0052009 | lld:lifeskim |
| pubmed-article:6329916 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6329916 | pubmed:dateCreated | 1984-7-27 | lld:pubmed |
| pubmed-article:6329916 | pubmed:abstractText | Direct selection of Kluyveromyces lactis resistant to the antibiotic G418 following transformation with the kanamycin resistance gene of Tn903 required the development of a procedure for producing high yields of viable spheroplasts and for the isolation of autonomous replication sequences (ARS). To obtain high yields of viable spheroplasts, cells were treated with (1) a thiol-reducing agent (L-cysteine), and (2) a high concentration of an osmotic stabilizer, 1.5 M sorbitol. Several ARS-containing plasmids were selected from a K. lactis recombinant DNA library in K. lactis and in Saccharomyces cerevisiae. Two of four ARS clones selected in K. lactis promoted transformation frequencies of 5-10 X 10(2) G418-resistant cells/micrograms of plasmid DNA. This frequency of transformation was at least twice as high as with ARS clones selected in S. cerevisiae. The stability of ARS-containing plasmids varied; after 20 generations of growth in the presence of G418, 16-38% of the cells remained resistant to the drug. In the absence of selection pressure less than 5% of the cells retained the drug-resistance phenotype. Plasmids containing the ARS1 or 2 mu replicon of S. cerevisiae failed to transform K. lactis for G418 resistance. Inclusion of S. cerevisiae centromere, CEN4, in a K. lactis ARS recombinant plasmid did not increase the stability of the plasmid in K. lactis, and marker genes on the vector segregated predominantly 4-:0+ through meiosis. We conclude that neither the ARS sequences or the centromere of S. cerevisiae was functioning in K. lactis. | lld:pubmed |
| pubmed-article:6329916 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6329916 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6329916 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6329916 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6329916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6329916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6329916 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6329916 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:6329916 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:6329916 | pubmed:author | pubmed-author:DicksonR CRC | lld:pubmed |
| pubmed-article:6329916 | pubmed:author | pubmed-author:SreekrishnaKK | lld:pubmed |
| pubmed-article:6329916 | pubmed:author | pubmed-author:WebsterT DTD | lld:pubmed |
| pubmed-article:6329916 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6329916 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:6329916 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6329916 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6329916 | pubmed:pagination | 73-81 | lld:pubmed |
| pubmed-article:6329916 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:6329916 | pubmed:meshHeading | pubmed-meshheading:6329916-... | lld:pubmed |
| pubmed-article:6329916 | pubmed:meshHeading | pubmed-meshheading:6329916-... | lld:pubmed |
| pubmed-article:6329916 | pubmed:meshHeading | pubmed-meshheading:6329916-... | lld:pubmed |
| pubmed-article:6329916 | pubmed:meshHeading | pubmed-meshheading:6329916-... | lld:pubmed |
| pubmed-article:6329916 | pubmed:meshHeading | pubmed-meshheading:6329916-... | lld:pubmed |
| pubmed-article:6329916 | pubmed:meshHeading | pubmed-meshheading:6329916-... | lld:pubmed |
| pubmed-article:6329916 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6329916 | pubmed:articleTitle | Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. | lld:pubmed |
| pubmed-article:6329916 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6329916 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:6329916 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6329916 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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