| pubmed-article:6309340 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0035542 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0046725 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:6309340 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:6309340 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:6309340 | pubmed:dateCreated | 1983-10-8 | lld:pubmed |
| pubmed-article:6309340 | pubmed:abstractText | A radiochemical method for the determination of the amino terminus on very small amounts (0.5-5 nmol) of protein is described. The high sensitivity of the method is achieved by using undiluted 1-fluoro-2,4-dinitro-[3,5-3H]benzene [( 3H]Dnp-F) as the labelling reagent under conditions in which a maximum amount of radioactive label is incorporated. Chemical homogeneity is achieved by reacting with excess unlabelled Dnp-F. High recovery is obtained by adding Dnp-albumin as carrier protein. A mixture of Dnp 14C-labelled amino acids is added prior to hydrolysis and identification of the amino terminus is made on the basis of the 3H/14C ratios of the separated Dnp-amino acids. The method was tested on insulin, pancreatic ribonuclease, and lysozyme which gave high 3H/14C ratios only in the expected amino-terminal amino acids. Application to multiple forms of poly(C)-avid ribonuclease gave only amino-terminal lysine. Two of four putative isozymes of 17 beta-hydroxysteroid dehydrogenase had serine as the amino terminus while the other two had aspartic acid or asparagine. | lld:pubmed |
| pubmed-article:6309340 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6309340 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6309340 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6309340 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:6309340 | pubmed:issn | 0714-7511 | lld:pubmed |
| pubmed-article:6309340 | pubmed:author | pubmed-author:KaplanHH | lld:pubmed |
| pubmed-article:6309340 | pubmed:author | pubmed-author:RabinE ZEZ | lld:pubmed |
| pubmed-article:6309340 | pubmed:author | pubmed-author:WilliamsonD... | lld:pubmed |
| pubmed-article:6309340 | pubmed:author | pubmed-author:TattrieBB | lld:pubmed |
| pubmed-article:6309340 | pubmed:author | pubmed-author:OdaGG | lld:pubmed |
| pubmed-article:6309340 | pubmed:author | pubmed-author:AntounG RGR | lld:pubmed |
| pubmed-article:6309340 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6309340 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:6309340 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6309340 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6309340 | pubmed:pagination | 323-7 | lld:pubmed |
| pubmed-article:6309340 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:meshHeading | pubmed-meshheading:6309340-... | lld:pubmed |
| pubmed-article:6309340 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6309340 | pubmed:articleTitle | A highly sensitive method for identification of amino termini of proteins: application to multiple forms of poly(C)-avid ribonuclease and 17 beta-hydroxysteroid dehydrogenase. | lld:pubmed |
| pubmed-article:6309340 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6309340 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:6309340 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6309340 | lld:pubmed |
