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pubmed-article:6298465pubmed:abstractTextCloned DNA copies of double-stranded RNA segments 7, 8, and 9 of UK bovine rotavirus were nick-translated with [alpha-(32)P]ATP and hybridized to double-stranded RNA of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o-aminophenylthioether paper. Specific hybridization of the UK calf clones to the separated RNA segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.lld:pubmed
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pubmed-article:6298465pubmed:articleTitleGene mapping of rotavirus double-stranded RNA segments by northern blot hybridization: application to segments 7, 8, and 9.lld:pubmed
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