| pubmed-article:6292832 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C0079866 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C0162735 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C1442161 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:6292832 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:6292832 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:6292832 | pubmed:dateCreated | 1983-1-19 | lld:pubmed |
| pubmed-article:6292832 | pubmed:abstractText | Deletion loop mutagenesis is a new, general method for site-directed mutagenesis that allows point mutations to the introduced within a sequence of DNA defined by a previously isolated deletion mutant. Wild type and deletion mutant DNA are cloned into a bacterial plasmid and each is cleaved with a different single cut restriction enzyme. Heteroduplexes are formed between the two DNAs to produce circular molecules containing a nick in each strand and a single-stranded deletion loop. The deletion loops are mutagenised using sodium bisulphite and the DNA transfected directly into a uracil repair deficient strain of Escherichia coli. Up to half of the resultant clones contain DNA produced by replication of the wild-type length strand and bear mutations exclusively within the target area. An example is given in which a deletion mutant lacking 21 nucleotides from the region coding for SV40 large-T was used. Eight of the possible nine target cytosine residues were mutagenised. The method described is specific, efficient and simple. | lld:pubmed |
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| pubmed-article:6292832 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6292832 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6292832 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6292832 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:6292832 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:6292832 | pubmed:author | pubmed-author:SmithA EAE | lld:pubmed |
| pubmed-article:6292832 | pubmed:author | pubmed-author:OostraB ABA | lld:pubmed |
| pubmed-article:6292832 | pubmed:author | pubmed-author:KalderonDD | lld:pubmed |
| pubmed-article:6292832 | pubmed:author | pubmed-author:ElyB KBK | lld:pubmed |
| pubmed-article:6292832 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6292832 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:6292832 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:6292832 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6292832 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6292832 | pubmed:pagination | 5161-71 | lld:pubmed |
| pubmed-article:6292832 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6292832 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6292832 | pubmed:articleTitle | Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants. | lld:pubmed |
| pubmed-article:6292832 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6292832 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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