| pubmed-article:6278496 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0025255 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C1179122 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0023768 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:6278496 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:6278496 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6278496 | pubmed:dateCreated | 1982-5-21 | lld:pubmed |
| pubmed-article:6278496 | pubmed:abstractText | Addition of membrane vesicles prepared from transverse tubule (T-tubule) membranes of rabbit skeletal muscle to the aqueous phase of a planar lipid bilayer induces a stepwise increase in conductance. This conductance is both voltage and Ca2+ dependent. At 1 mM Ca2+, the steady-state conductance is maximal at voltages higher than +20 mV and decreases for more negative voltages. (Voltages refer to the side to which the vesicles are added, cis) Decreasing the Ca2+ concentration reversibly shifts the conductance-voltage curve toward the right along the voltage axis. Furthermore, Ca2+ can activate the conductance only if added to the cis compartment. Neither Mg2+, Ba2+, nor Cd2+ can activate the conductance induced by T-tubule vesicles. Addition of 5 mM tetraethylammonium ion to the trans, but not the cis, side abolishes the T-tubule-induced conductance. The Ca2+-dependent conductance appears as a consequence of ionic channel formation. Single-channel activity appears in bursts followed by periods of time in which the channel remains "silent". The conductance of the open channel averages 226 pS in 0.1 M KC1 and is voltage and Ca2+ independent. However, the fraction of time that the channel remains in the open state is voltage and Ca2+ dependent in a manner that parallels the voltage and Ca2+ dependence of the multichannel membrane. The channel is 6.6 times more permeable to K+ than to Na+ and is impermeable to C1-. | lld:pubmed |
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| pubmed-article:6278496 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6278496 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6278496 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6278496 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:6278496 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6278496 | pubmed:author | pubmed-author:HidalgoCC | lld:pubmed |
| pubmed-article:6278496 | pubmed:author | pubmed-author:LatorreRR | lld:pubmed |
| pubmed-article:6278496 | pubmed:author | pubmed-author:VergaraCC | lld:pubmed |
| pubmed-article:6278496 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6278496 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:6278496 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6278496 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6278496 | pubmed:pagination | 805-9 | lld:pubmed |
| pubmed-article:6278496 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6278496 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6278496 | pubmed:articleTitle | Reconstitution in planar lipid bilayers of a Ca2+-dependent K+ channel from transverse tubule membranes isolated from rabbit skeletal muscle. | lld:pubmed |
| pubmed-article:6278496 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6278496 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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