| pubmed-article:6271056 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6271056 | lifeskim:mentions | umls-concept:C0009063 | lld:lifeskim |
| pubmed-article:6271056 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:6271056 | lifeskim:mentions | umls-concept:C0005390 | lld:lifeskim |
| pubmed-article:6271056 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6271056 | pubmed:dateCreated | 1981-12-15 | lld:pubmed |
| pubmed-article:6271056 | pubmed:abstractText | Thirty-five strains of Clostridium perfringens were examined for their ability to transform bile acids, both in growing cultures and by washed whole cells. All of the strains oxidized the 3 alpha-hydroxy group to an oxo group, and all except three converted the same alpha-hydroxy group into a beta-configuration. The oxidative 3 alpha-dehydrogenation was barely detectable under anaerobic cultural conditions but was clearly demonstrated in an aerated system using washed whole cells, with a pH optimum between 7.0 and 9.0. The epimerizing reaction amounting to 10 to 20% conversion was observed in anaerobic cultures and also with resting cells, irrespective of oxygen supply. Both reactions were carried out with seven conventional 3 alpha-hydroxy bile acids, thus producing a series of 3-oxo and 3 beta-hydroxy derivatives that could be examined for gas-liquid chromatographic and mass spectrometric behavior. No evidence for the occurrence of 7 alpha- and 12 alpha-hydroxysteroid dehydrogenase activities among the test strains was found. A highly potent deconjugating hydrolase was elaborated by all of the strains. | lld:pubmed |
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| pubmed-article:6271056 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6271056 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6271056 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6271056 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6271056 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:6271056 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:6271056 | pubmed:author | pubmed-author:HiranoSS | lld:pubmed |
| pubmed-article:6271056 | pubmed:author | pubmed-author:MasudaNN | lld:pubmed |
| pubmed-article:6271056 | pubmed:author | pubmed-author:MukaiHH | lld:pubmed |
| pubmed-article:6271056 | pubmed:author | pubmed-author:OdaHH | lld:pubmed |
| pubmed-article:6271056 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6271056 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:6271056 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6271056 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6271056 | pubmed:pagination | 394-9 | lld:pubmed |
| pubmed-article:6271056 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6271056 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6271056 | pubmed:articleTitle | Transformation of bile acids by Clostridium perfringens. | lld:pubmed |
| pubmed-article:6271056 | pubmed:publicationType | Journal Article | lld:pubmed |
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