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| pubmed-article:6268841 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6268841 | pubmed:dateCreated | 1981-11-18 | lld:pubmed |
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| pubmed-article:6268841 | pubmed:abstractText | The complete nucleotide sequences of the vesicular stomatitis virus (VSV) mRNA's encoding the N and NS proteins have been determined from the sequences of cDNA clones. The mRNA encoding the N protein is 1,326 nucleotides long, excluding polyadenylic acid. It contains an open reading frame for translation which extends from the 5'-proximal AUG codon to encode a protein of 422 amino acids. The N and mRNA is known to contain a major ribosome binding site at the 5'-proximal AUG codon and two other minor ribosome binding sites. These secondary sites have been located unambiguously at the second and third AUG codons in the N mRNA sequence. Translational initiation at these sites, if it in fact occurs, would result in synthesis of two small proteins in a second reading frame. The VSV and mrna encoding the NS protein is 815 nucleotides long, excluding polyadenylic acid, and encodes a protein of 222 amino acids. The predicted molecular weight of the NS protein (25,110) is approximately one-half of that predicted from the mobility of NS protein on sodium dodecyl sulfate-polyacrylamide gels. Deficiency of sodium dodecyl sulfate binding to a large negatively charged domain in the NS protein could explain this anomalous electrophoretic mobility. | lld:pubmed |
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| pubmed-article:6268841 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6268841 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6268841 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6268841 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:6268841 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:6268841 | pubmed:author | pubmed-author:RoseJ KJK | lld:pubmed |
| pubmed-article:6268841 | pubmed:author | pubmed-author:IversonL ELE | lld:pubmed |
| pubmed-article:6268841 | pubmed:author | pubmed-author:GreeneJ RJR | lld:pubmed |
| pubmed-article:6268841 | pubmed:author | pubmed-author:GallioneC JCJ | lld:pubmed |
| pubmed-article:6268841 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6268841 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:6268841 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6268841 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6268841 | pubmed:pagination | 529-35 | lld:pubmed |
| pubmed-article:6268841 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6268841 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6268841 | pubmed:articleTitle | Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus N and NS proteins. | lld:pubmed |
| pubmed-article:6268841 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6268841 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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