| pubmed-article:6267604 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0450429 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0017366 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C0085431 | lld:lifeskim |
| pubmed-article:6267604 | lifeskim:mentions | umls-concept:C1704222 | lld:lifeskim |
| pubmed-article:6267604 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:6267604 | pubmed:dateCreated | 1981-10-29 | lld:pubmed |
| pubmed-article:6267604 | pubmed:abstractText | By in vitro recombination we have constructed hybrid plasmids capable of complementing a conditional lethal mutator mutation, dnaQ49, in Escherichia coli K12. The dnaQ+ plasmids consist of a full-length pBR322 DNA and a 1.5-kilobase DNA fragment derived from the E. coli chromosome. Specific labeling of plasmid-encoded proteins by the maxicell method revealed that the 1.5-kilobase insert codes for two proteins, one whose molecular weight is 25,000 [the 25-kilodalton (kDal) protein] and the other whose molecular weight is 21,000 (the 21-kDal protein). Because insertion of gamma delta sequence into the dnaQ gene of the plasmid resulted in disappearance of the 25-kDal protein, it was concluded that the 25-kDal protein is the dnaQ gene product. The 21-kDal protein was identified as RNase H on the basis of the following evidence. (i) Cells harboring the dnaQ+ plasmids, with or without the gamma delta insertion in the dnaQ gene, had a 5- to 7-fold higher level of RNase H activity than cells harboring pBR322. (ii) After induction of cells that are lysogenized with dnaQ+-transducing lambda phages, RNase H activity increased considerably. A similar high level of RNase H activity was observed with transducing phages whose dnaQ function was inactivated by insertion of a transposon, Tn3, into the gene, (iii) The plasmid-encoded RNase H, labeled with [35S]methionine, was purified in a manner essentially similar to that of the chromosome-encoded enzyme. These results suggest that the dnaQ gene and the structural gene for RNase H, termed gene rnh, are closely linked and located at 5 min on the linkage map. | lld:pubmed |
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| pubmed-article:6267604 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6267604 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6267604 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6267604 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6267604 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:6267604 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6267604 | pubmed:author | pubmed-author:SekiguchiMM | lld:pubmed |
| pubmed-article:6267604 | pubmed:author | pubmed-author:MaruyamaMM | lld:pubmed |
| pubmed-article:6267604 | pubmed:author | pubmed-author:MakiHH | lld:pubmed |
| pubmed-article:6267604 | pubmed:author | pubmed-author:HoriuchiTT | lld:pubmed |
| pubmed-article:6267604 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6267604 | pubmed:volume | 78 | lld:pubmed |
| pubmed-article:6267604 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6267604 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6267604 | pubmed:pagination | 3770-4 | lld:pubmed |
| pubmed-article:6267604 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:6267604 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6267604 | pubmed:articleTitle | Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes. | lld:pubmed |
| pubmed-article:6267604 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6267604 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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