| pubmed-article:6265450 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0017687 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:6265450 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:6265450 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:6265450 | pubmed:dateCreated | 1981-9-15 | lld:pubmed |
| pubmed-article:6265450 | pubmed:abstractText | The effects of insulin and glucagon on the (Na+-K+)-ATPase transport activity in freshly isolated rat hepatocytes were investigated by measuring the ouabain-sensitive, active uptake of 86Rb+. The active uptake of 86Rb+ was increased by 18% (p less than 0.05) in the presence of 100 nM insulin, and by 28% (p less than 0.005) in the presence of nM glucagon. These effects were detected as early as 2 min after hepatocyte exposure to either hormone. Half-maximal stimulation was observed with about 0.5 nm insulin and 0.3 nM glucagon. The stimulation of 86Rb+ uptake by insulin occurred in direct proportion to the steady state occupancy of a high affinity receptor by the hormone (the predominant insulin-binding species in hepatocytes at 37 degrees C. For glucagon, half-maximal response was obtained with about 5% of the total receptors occupied by the hormone. Amiloride (a specific inhibitor of Na+ influx) abolished the insulin stimulation of 86Rb+ uptake while inhibiting that of glucagon only partially. Accordingly, insulin was found to rapidly enhance the initial rate of 22Na+ uptake, whereas glucagon had no detectable effect on 22Na+ influx. These results indicate that monovalent cation transport is influenced by insulin and glucagon in isolated rat hepatocytes. In contrast to glucagon, which appears to enhance 86Rb+ influx through the (Na+-K+)-ATPase without affecting Na+ influx, insulin stimulates Na+ entry which in turn may increase the pump activity by increasing the availability of Na+ ions to internal Na+ transport sites of the (Na+-K+)-ATPase. | lld:pubmed |
| pubmed-article:6265450 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6265450 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6265450 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6265450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:6265450 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6265450 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:6265450 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:6265450 | pubmed:author | pubmed-author:FreychetPP | lld:pubmed |
| pubmed-article:6265450 | pubmed:author | pubmed-author:FehlmannMM | lld:pubmed |
| pubmed-article:6265450 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6265450 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:6265450 | pubmed:volume | 256 | lld:pubmed |
| pubmed-article:6265450 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6265450 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6265450 | pubmed:pagination | 7449-53 | lld:pubmed |
| pubmed-article:6265450 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:6265450 | pubmed:meshHeading | pubmed-meshheading:6265450-... | lld:pubmed |
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| pubmed-article:6265450 | pubmed:meshHeading | pubmed-meshheading:6265450-... | lld:pubmed |
| pubmed-article:6265450 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6265450 | pubmed:articleTitle | Insulin and glucagon stimulation of (Na+-K+)-ATPase transport activity in isolated rat hepatocytes. | lld:pubmed |
| pubmed-article:6265450 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6265450 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:6265450 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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