| pubmed-article:6247281 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6247281 | lifeskim:mentions | umls-concept:C0877837 | lld:lifeskim |
| pubmed-article:6247281 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:6247281 | lifeskim:mentions | umls-concept:C0872054 | lld:lifeskim |
| pubmed-article:6247281 | lifeskim:mentions | umls-concept:C0028602 | lld:lifeskim |
| pubmed-article:6247281 | lifeskim:mentions | umls-concept:C0449851 | lld:lifeskim |
| pubmed-article:6247281 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6247281 | pubmed:dateCreated | 1980-8-28 | lld:pubmed |
| pubmed-article:6247281 | pubmed:abstractText | The technique of nucleic acid hybridization was used to detect the presence of murine cytomegalovirus (MCMV)-specific deoxyribonucleic acid (DNA) in cell cultures and salivary gland tissues. The presence of approximately 4.5 and 0.2 genome equivalents per cell of MCMV-specific DNA was identified in cultures of salivary (ISG2) and prostate gland (IP) cells, respectively. These cells, derived from animals with experimentally induced latent infections, were negative for virus-specific antigens by immunofluorescence and on electron microscopy revealed no visible evidence of the presence of herpesviruses. A cell line derived from the salivary gland of an uninoculated animal (NSG2) was also found to possess MCMV-specific DNA (0.2 genome equivalents per cell). For this reason, salivary gland tissues from uninoculated animals supplied as "specific pathogen-free" mice by three commercial sources were tested upon arrival for the presence of MCMC-specific DNA. MCMV-specific DNA was detectable in pooled salivary gland extracts from uninoculated animals derived from two commercial sources. All of these animals were seronegative and virus negative by conventional infectivity assays. | lld:pubmed |
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| pubmed-article:6247281 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6247281 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6247281 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6247281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6247281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6247281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6247281 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6247281 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6247281 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:6247281 | pubmed:author | pubmed-author:HuangE SES | lld:pubmed |
| pubmed-article:6247281 | pubmed:author | pubmed-author:LaneD HDH | lld:pubmed |
| pubmed-article:6247281 | pubmed:author | pubmed-author:CheungK SKS | lld:pubmed |
| pubmed-article:6247281 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6247281 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:6247281 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6247281 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6247281 | pubmed:pagination | 851-4 | lld:pubmed |
| pubmed-article:6247281 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:6247281 | pubmed:meshHeading | pubmed-meshheading:6247281-... | lld:pubmed |
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| pubmed-article:6247281 | pubmed:meshHeading | pubmed-meshheading:6247281-... | lld:pubmed |
| pubmed-article:6247281 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6247281 | pubmed:articleTitle | Murine cytomegalovirus: detection of latent infection by nucleic acid hybridization technique. | lld:pubmed |
| pubmed-article:6247281 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6247281 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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