| pubmed-article:6243775 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6243775 | lifeskim:mentions | umls-concept:C0012940 | lld:lifeskim |
| pubmed-article:6243775 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:6243775 | lifeskim:mentions | umls-concept:C0005863 | lld:lifeskim |
| pubmed-article:6243775 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6243775 | pubmed:dateCreated | 1980-4-17 | lld:pubmed |
| pubmed-article:6243775 | pubmed:abstractText | A method, called "protein blotting," for the detection of DNA-binding proteins is described. Proteins are separated on an SDA-polyacrylamide gel. The gel is sandwiched between 2 nitrocellulose filters and the proteins allowed to diffuse out of the gel and onto the filters. The proteins are tightly bound to each filter, producing a replica of the original gel pattern. The replica is used to detect DNA-binding proteins, RNA-binding proteins or histone-binding proteins by incubation of the filter with [32P]DNA, [125I]RNA, or [125I] histone. Evidence is also presented that specific protein-DNA interactions may be detected by this technique; under appropriate conditions, the lac repressor binds only to DNA containing the lac operator. Strategies for the detection of specific protein-DNA interactions are discussed. | lld:pubmed |
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| pubmed-article:6243775 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6243775 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6243775 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6243775 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:6243775 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:6243775 | pubmed:author | pubmed-author:LaemmliU KUK | lld:pubmed |
| pubmed-article:6243775 | pubmed:author | pubmed-author:BowerFF | lld:pubmed |
| pubmed-article:6243775 | pubmed:author | pubmed-author:SteinbergJJ | lld:pubmed |
| pubmed-article:6243775 | pubmed:author | pubmed-author:WeintraubHH | lld:pubmed |
| pubmed-article:6243775 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6243775 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:6243775 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:6243775 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6243775 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6243775 | pubmed:pagination | 1-20 | lld:pubmed |
| pubmed-article:6243775 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6243775 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6243775 | pubmed:articleTitle | The detection of DNA-binding proteins by protein blotting. | lld:pubmed |
| pubmed-article:6243775 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6243775 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6243775 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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