| pubmed-article:6239615 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C1622418 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C0184661 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C1148926 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:6239615 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:6239615 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6239615 | pubmed:dateCreated | 1985-1-9 | lld:pubmed |
| pubmed-article:6239615 | pubmed:abstractText | A technique is described for the isolation of a plasma membrane-enriched preparation from a rat liver post-mitochondrial fraction by using discontinuous Percoll density-gradient centrifugation. The procedure is simple, of high reproducibility and yield and requires a total isolation time of only 90 min. The preparation consists almost exclusively of membrane vesicles and is enriched approx. 26-fold in plasma membrane-localized enzymes with minor contamination (less than 10%) with membranes derived mainly from the endoplasmic reticulum and Golgi apparatus. Approx. 20% of the fraction comprises tightly-sealed vesicles in the inverted orientation which are capable of accumulating calcium ions and exhibiting vanadate-insensitive Ca2+-ATPase activity. The properties of these activities, including insensitivity to vanadate, oxalate, and to p-chloromercuribenzoate as well as a lack of requirement for added Mg2+, contrast markedly with the reported properties of Ca2+ transport by the endoplasmic reticulum isolated from rat liver. The technique may have wide application in the study of plasma membrane-associated activities in rat liver, particularly in relation to sinusoidal membrane surface-related events. | lld:pubmed |
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| pubmed-article:6239615 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6239615 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6239615 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6239615 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6239615 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:6239615 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:6239615 | pubmed:author | pubmed-author:BygraveF LFL | lld:pubmed |
| pubmed-article:6239615 | pubmed:author | pubmed-author:EppingR JRJ | lld:pubmed |
| pubmed-article:6239615 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6239615 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:6239615 | pubmed:volume | 223 | lld:pubmed |
| pubmed-article:6239615 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6239615 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6239615 | pubmed:pagination | 733-45 | lld:pubmed |
| pubmed-article:6239615 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:6239615 | pubmed:meshHeading | pubmed-meshheading:6239615-... | lld:pubmed |
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| pubmed-article:6239615 | pubmed:meshHeading | pubmed-meshheading:6239615-... | lld:pubmed |
| pubmed-article:6239615 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6239615 | pubmed:articleTitle | A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibiting Ca2+-transport and Ca2+-ATPase activities. | lld:pubmed |
| pubmed-article:6239615 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6239615 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:6239615 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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