| pubmed-article:6234201 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C0031298 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C0021745 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:6234201 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:6234201 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6234201 | pubmed:dateCreated | 1984-7-27 | lld:pubmed |
| pubmed-article:6234201 | pubmed:abstractText | Several recombinant plasmids have been constructed which direct high-level synthesis of mature human interferon gamma (IFN-gamma) in Escherichia coli using the inducible leftward promoter pL of phage lambda followed by a translational initiator region derived either from the phage MS2 replicase gene or the E. coli tryptophan attenuator region. Under these conditions, IFN levels of up to 25% of the total cellular protein can be achieved. The highest levels were obtained when a terminator of transcription was cloned downstream from the IFN-gamma sequence. IFN-gamma was almost entirely found in the initial pellet fraction and not in soluble extracts. Co-induction of the lysis genes derived from phage MS2 or from phage lambda, inserted downstream from the IFN-gamma sequence, did not enhance the biological activity present in the supernatant fraction. | lld:pubmed |
| pubmed-article:6234201 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6234201 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6234201 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6234201 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6234201 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6234201 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6234201 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:6234201 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:6234201 | pubmed:author | pubmed-author:DevosRR | lld:pubmed |
| pubmed-article:6234201 | pubmed:author | pubmed-author:FiersWW | lld:pubmed |
| pubmed-article:6234201 | pubmed:author | pubmed-author:AlleyGG | lld:pubmed |
| pubmed-article:6234201 | pubmed:author | pubmed-author:RemautEE | lld:pubmed |
| pubmed-article:6234201 | pubmed:author | pubmed-author:SimonsGG | lld:pubmed |
| pubmed-article:6234201 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6234201 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:6234201 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6234201 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6234201 | pubmed:pagination | 55-64 | lld:pubmed |
| pubmed-article:6234201 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:meshHeading | pubmed-meshheading:6234201-... | lld:pubmed |
| pubmed-article:6234201 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6234201 | pubmed:articleTitle | High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda. | lld:pubmed |
| pubmed-article:6234201 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6234201 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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