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pubmed-article:6202757pubmed:abstractTextMononuclear cells were isolated from the spinal cords of 13 Lewis rats with clinical EAE induced by immunization with MBP in complete Freund's adjuvant. MBP-reactive lymphocytes were recovered from nine of these 13 SC-MNC populations by in vitro culture with MBP, followed by propagation of responding lymphocytes in IL 2-containing medium. T cell subset phenotype determination indicated a predominance of helper phenotype T cells in each of the two cultures examined. Clinical EAE was induced in naive Lewis rats by adoptive transfer of MBP-reactive lymphocytes recovered from three of these cultures.lld:pubmed
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pubmed-article:6202757pubmed:articleTitleRecovery of myelin basic protein reactive T cells from spinal cords of Lewis rats with autoimmune encephalomyelitis.lld:pubmed
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