| pubmed-article:6185479 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0079382 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0087111 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0010531 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0043314 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:6185479 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:6185479 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6185479 | pubmed:dateCreated | 1983-3-11 | lld:pubmed |
| pubmed-article:6185479 | pubmed:abstractText | Friend erythroleukemic cells contain both type I and type II cAMP-dependent protein kinases. The amounts of type I and type II regulatory subunits (R I and R II) were established by a combination of the equilibrium binding of cyclic [3H]AMP and specific immunoprecipitation. In control cells the total specific cAMP-binding activity was 9.8 pmol/mg of cytosolic protein and the ratio of R II to R I was 1.2. After the induction of differentiation by growth in 2% dimethyl sulfoxide, the total cAMP-binding capacity doubled, but larger and opposing changes in R II and R I levels were observed. The concentration of R II tripled (to 16.6 pmol/mg) while R I content (1.5 pmol/mg) declined to one-third of the control cell level, resulting in an R II:R I value of 11.1. Treatment of Friend cells with 0.5 mM 8-bromoadenosine 3':5'-monophosphate (8-Br-cAMP) and 0.2 mM methylisobutyl xanthine for 2 days did not stimulate cell differentiation. however, the cAMP-binding capacity nearly doubled and the cells accumulated a high level of R II while R I declined. This resulted in a distribution of R subunits closely resembling that of differentiated cells. Cyclic AMP-stimulated histone kinase activity increased in proportion to cAMP-binding activity during differentiation; no such increase was seen following 8-Br-cAMP treatment. Fractionation of cytosolic extracts by DEAE-cellulose chromatography revealed that both R I and R II were fully associated with the catalytic subunit (C) of protein kinase before and after differentiation. In extracts of 8-Br-cAMP-treated cells all of R I and 40 to 60% of R II were not associated with C. Free R II isolated from these cells was indistinguishable from RII present in holoenzyme. Thus, erythroid differentiation of Friend cells results in a coordinate accumulation of R II and C subunits as well as a decline in R I content. In contrast, chronic exposure to 8-Br-cAMP elicits similar changes in R subunit levels but disrupts the coordinate regulation of R and C content. | lld:pubmed |
| pubmed-article:6185479 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6185479 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6185479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6185479 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6185479 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:6185479 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:6185479 | pubmed:author | pubmed-author:RubinC SCS | lld:pubmed |
| pubmed-article:6185479 | pubmed:author | pubmed-author:SchwartzD ADA | lld:pubmed |
| pubmed-article:6185479 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6185479 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:6185479 | pubmed:volume | 258 | lld:pubmed |
| pubmed-article:6185479 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6185479 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6185479 | pubmed:pagination | 777-84 | lld:pubmed |
| pubmed-article:6185479 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:6185479 | pubmed:meshHeading | pubmed-meshheading:6185479-... | lld:pubmed |
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| pubmed-article:6185479 | pubmed:meshHeading | pubmed-meshheading:6185479-... | lld:pubmed |
| pubmed-article:6185479 | pubmed:meshHeading | pubmed-meshheading:6185479-... | lld:pubmed |
| pubmed-article:6185479 | pubmed:meshHeading | pubmed-meshheading:6185479-... | lld:pubmed |
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| pubmed-article:6185479 | pubmed:meshHeading | pubmed-meshheading:6185479-... | lld:pubmed |
| pubmed-article:6185479 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6185479 | pubmed:articleTitle | Regulation of cAMP-dependent protein kinase subunit levels in Friend erythroleukemic cells. Effects of differentiation and treatment with 8-Br-cAMP and methylisobutyl xanthine. | lld:pubmed |
| pubmed-article:6185479 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6185479 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6185479 | lld:pubmed |
