| pubmed-article:6173214 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6173214 | lifeskim:mentions | umls-concept:C1564874 | lld:lifeskim |
| pubmed-article:6173214 | lifeskim:mentions | umls-concept:C0035736 | lld:lifeskim |
| pubmed-article:6173214 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:6173214 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:6173214 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6173214 | pubmed:dateCreated | 1982-4-12 | lld:pubmed |
| pubmed-article:6173214 | pubmed:abstractText | We have studied RNA transcripts of the defective parvovirus, adeno-associated virus (AAV) present in poly(A)rich and poly-(A)free fractions of nuclear and cytoplasmic RNA prepared from cells infected together with a helper adenovirus. Cytoplasmic poly-(A)rich RNA contains three overlapping spliced AAV RNAs having sizes of 3.9 X 10(3), 3.3 X 10(3) and 2.3 X 10(3) bases respectively. The nuclear precursors of these RNAs appear to be the coterminal unspliced poly(A)-rich RNAs containing 4.2 X 10(3), 3.6 X 10(3) and 2.6 X 10(3) bases respectively. These unspliced RNAs were also found in the cytoplasm. The nuclear poly(A)-free RNA contained a heterogenous population of AAV RNAs that were generally smaller than 2.3 X 10(3) bases. In addition, the Hirt pellet fraction of the nuclear RNA contained two discrete AAV poly(A)-free RNAs having sizes of 2.5 X 10(3) and 2.8 X 10(3) bases. The 4.2 X 10(3) and 3.6 X 10(3)-base unspliced RNAs are more abundant than the coterminal 3.9 X 10(3) and 3.3 X 10(3)-base spliced RNAs whereas the 2.3 X 10(3)-base spliced RNA is much more abundant than the 2.6 X 10(3)-base unspliced RNA. Thus, the cytoplasmic abundance of the AAV spliced RNAs appears to be controlled in part by the post-transcriptional events of splicing or message stability. We also analysed the effects of AAV defective-interfering genomes upon AAV transcription. These studies showed that when synthesis of standard AAV genomes was inhibited more than 10-fold by defective-interfering genomes there was no significant effect on the types or amounts of AAV RNA transcripts which accumulated. These observations indicate that interference by defective-interfering genomes occurs mostly at the level of DNA replication rather than transcription. | lld:pubmed |
| pubmed-article:6173214 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6173214 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6173214 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6173214 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6173214 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:6173214 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:6173214 | pubmed:author | pubmed-author:MarcusC JCJ | lld:pubmed |
| pubmed-article:6173214 | pubmed:author | pubmed-author:LaughlinC ACA | lld:pubmed |
| pubmed-article:6173214 | pubmed:author | pubmed-author:CarterB JBJ | lld:pubmed |
| pubmed-article:6173214 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6173214 | pubmed:volume | 121 | lld:pubmed |
| pubmed-article:6173214 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6173214 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6173214 | pubmed:pagination | 147-54 | lld:pubmed |
| pubmed-article:6173214 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:6173214 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6173214 | pubmed:articleTitle | Adeno-associated virus RNA transcription in vivo. | lld:pubmed |
| pubmed-article:6173214 | pubmed:publicationType | Journal Article | lld:pubmed |
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