| pubmed-article:6166921 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6166921 | lifeskim:mentions | umls-concept:C1522642 | lld:lifeskim |
| pubmed-article:6166921 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:6166921 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:6166921 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
| pubmed-article:6166921 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:6166921 | pubmed:dateCreated | 1981-9-15 | lld:pubmed |
| pubmed-article:6166921 | pubmed:abstractText | A method for cloning mRNAs has been used which results in a high yield of recombinants containing complete 5'-terminal mRNA sequences. It is not dependent on self-priming to generate double-stranded DNA and therefore the S1 nuclease digestion step is not required. Instead, the cDNA is dCMP-tailed at its 3'-end with terminal deoxynucleotidyl transferase (TdT). The synthesis of the second strand is primed by oligo(dG) hybridized to the 3'-tail. Double-stranded cDNA is subsequently tailed with dCTP and annealed to dGMP-tailed vector DNA. This approach overcomes the loss of the 5'-terminal mRNA sequences and the problem of artifacts which may be introduced into cloned cDNA sequences. Chicken lysozyme cDNA was cloned into pBR322 by this procedure with a transformation efficiency of 5 x 10(3) recombinant clones per ng of ds-cDNA. Sequence analysis revealed that at least nine out of nineteen randomly isolated plasmids contained the entire 5'-untranslated mRNA sequence. The data strongly support the conclusion that the 5'-untranslated region of the lysozyme mRNA is heterogeneous in length. | lld:pubmed |
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| pubmed-article:6166921 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6166921 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6166921 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6166921 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6166921 | pubmed:month | May | lld:pubmed |
| pubmed-article:6166921 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:6166921 | pubmed:author | pubmed-author:HauserHH | lld:pubmed |
| pubmed-article:6166921 | pubmed:author | pubmed-author:LangMM | lld:pubmed |
| pubmed-article:6166921 | pubmed:author | pubmed-author:SchützGG | lld:pubmed |
| pubmed-article:6166921 | pubmed:author | pubmed-author:LindenmaierWW | lld:pubmed |
| pubmed-article:6166921 | pubmed:author | pubmed-author:GrezMM | lld:pubmed |
| pubmed-article:6166921 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6166921 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:6166921 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:6166921 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6166921 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6166921 | pubmed:pagination | 2251-66 | lld:pubmed |
| pubmed-article:6166921 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6166921 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6166921 | pubmed:articleTitle | 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. | lld:pubmed |
| pubmed-article:6166921 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6166921 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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