| pubmed-article:6155477 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6155477 | lifeskim:mentions | umls-concept:C0038975 | lld:lifeskim |
| pubmed-article:6155477 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:6155477 | lifeskim:mentions | umls-concept:C0003343 | lld:lifeskim |
| pubmed-article:6155477 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:6155477 | lifeskim:mentions | umls-concept:C2348205 | lld:lifeskim |
| pubmed-article:6155477 | lifeskim:mentions | umls-concept:C0023043 | lld:lifeskim |
| pubmed-article:6155477 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6155477 | pubmed:dateCreated | 1980-8-25 | lld:pubmed |
| pubmed-article:6155477 | pubmed:abstractText | We have isolated three clones of hybrid cells which synthesize antibodies specific for determinants on simian virus 40 (SV40) T antigens. Mouse myeloma NS1 cells were fused with spleen cells from mice that had been immunized with SV40-transformed mouse cells. Hybrid cells were selected in HAT medium and cloned in soft agar. We used an enzyme-linked immunosorbent assay for detection and quantification of mouse antibodies against SV40 T antigens. Monoclonal antibodies from 3 of the 24 clones that scored as positive in the enzyme-linked immunosorbent assay were verified by immunoprecipitation to be specific for SV40 T antigens. Two clones (7 and 412) produced antibodies that recognized denaturation-sensitive antigenic determinants unique to large T antigen. Antibodies from clone 7 appeared to have a low affinity for large T antigen. Antibodies from clone 412 had a higher affinity for large T antigen but did not recognize a subclass of large T antigen that was recognized by tumor serum. Antibodies of the third clone, clone 122, recognized a denaturation-stable antigenic determinant of the 53,000-dalton mouse nonviral T antigen in SV40-transformed cells. Antibodies from clone 122 also recognized similar (51,000- to 56,000-dalton) nonviral T antigens in SV40-transormed or lytically infected cells from five mammalian species and in four uninfected mouse lines. From these observations, we have concluded that (i) the 94,000-dalton SV40 large T antigen may exist as immunologically distinguishable subclasses, and (ii) the nonviral T antigens of five mammalian species share at least one antigenic determinant. | lld:pubmed |
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| pubmed-article:6155477 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6155477 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6155477 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6155477 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6155477 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:6155477 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:6155477 | pubmed:author | pubmed-author:FennoJJ | lld:pubmed |
| pubmed-article:6155477 | pubmed:author | pubmed-author:GurneyE GEG | lld:pubmed |
| pubmed-article:6155477 | pubmed:author | pubmed-author:HarrisonR ORO | lld:pubmed |
| pubmed-article:6155477 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6155477 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:6155477 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6155477 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6155477 | pubmed:pagination | 752-63 | lld:pubmed |
| pubmed-article:6155477 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6155477 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6155477 | pubmed:articleTitle | Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. | lld:pubmed |
| pubmed-article:6155477 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6155477 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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