| pubmed-article:6146633 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6146633 | lifeskim:mentions | umls-concept:C0008155 | lld:lifeskim |
| pubmed-article:6146633 | lifeskim:mentions | umls-concept:C1260875 | lld:lifeskim |
| pubmed-article:6146633 | lifeskim:mentions | umls-concept:C0007004 | lld:lifeskim |
| pubmed-article:6146633 | lifeskim:mentions | umls-concept:C0009630 | lld:lifeskim |
| pubmed-article:6146633 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:6146633 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
| pubmed-article:6146633 | pubmed:dateCreated | 1984-9-14 | lld:pubmed |
| pubmed-article:6146633 | pubmed:abstractText | Cell surface carbohydrates, detected by fluorescein isothiocyanate/concanavalin A (FITC-ConA), were identified at four locations on gametes of Chlamydomonas reinhardtii. (1) The cell wall: uniform labelling with FITC-ConA was observed; a substantial number of sites were localized in the sodium dodecyl sulphate-insoluble inner wall, which contains the flagellar collars. (2) The periplasm: a crescent-shaped area was visualized with FITC-ConA and localized by ferritin-ConA. We were able to recover autolytic activity on a ConA affinity column from the mating medium of wild-type cells after the release of these periplasmic sites. The cell-wall-less mutant CW15 displays no periplasmic sites and demonstrates a corresponding inability to release autolytic activity after mating for 60 min. A model for wall lysis is presented, which considers the involvement of these sites in the lytic process. (3) The mating structure: during mating a small fluorescent plaque-like site was observed on cells at a location corresponding to the carbohydrate-like zone of the mating type minus mating structure and may indicate the involvement of ConA binding material in gametic cell fusion. (4) Secreted products: following cell fusion zygotes begin to secrete ConA positive material at about 1 1/2 h. After 24 h a ConA positive zygote wall and pellicle appear. | lld:pubmed |
| pubmed-article:6146633 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6146633 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6146633 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6146633 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6146633 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6146633 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6146633 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6146633 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6146633 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6146633 | pubmed:issn | 0021-9533 | lld:pubmed |
| pubmed-article:6146633 | pubmed:author | pubmed-author:WeissR LRL | lld:pubmed |
| pubmed-article:6146633 | pubmed:author | pubmed-author:MillikinB EBE | lld:pubmed |
| pubmed-article:6146633 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6146633 | pubmed:volume | 66 | lld:pubmed |
| pubmed-article:6146633 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6146633 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6146633 | pubmed:pagination | 223-39 | lld:pubmed |
| pubmed-article:6146633 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:meshHeading | pubmed-meshheading:6146633-... | lld:pubmed |
| pubmed-article:6146633 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6146633 | pubmed:articleTitle | Distribution of concanavalin a binding carbohydrates during mating in Chlamydomonas. | lld:pubmed |
| pubmed-article:6146633 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6146633 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6146633 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6146633 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6146633 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6146633 | lld:pubmed |
