| pubmed-article:6128311 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0031921 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0021075 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0205148 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:6128311 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:6128311 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6128311 | pubmed:dateCreated | 1983-1-19 | lld:pubmed |
| pubmed-article:6128311 | pubmed:abstractText | Type 1 fimbriae from two strains of Escherichia coli, K-12-derived CSH50 and a clinical isolate VL-2, were purified by a simplified procedure, which should be applicable to a variety of bacterial strains. After mechanical removal from the cells, the fimbriae were sedimented in the ultracentrifuge and resuspended in 5 M urea to disaggregate cell membranes and flagella, leaving the urea-resistant fimbriae intact. After several hours at 37 degrees C, this crude fimbrial suspension was diluted to 1 M urea, and the intact fimbriae were sedimented through a 1 M urea-1 M sucrose cushion. The pellet was found to be pure fimbriae by sodium docecyl sulfate-polyacrylamide gel electrophoresis, with apparent subunit molecular weights of 17,000 for the fimbriae from K-12 strain CSH50 and 19,000 for those from the clinical isolate VL-2. High-titer rabbit antiserum raised against CSH50 fimbriae was specific for fimbriae by indirect ferritin labeling and immunoprecipitation and was used to develop an enzyme-linked immunosorbent assay. Competitive inhibition of antifimbrial antiserum in the enzyme-linked immunosorbent assay by a known amount of either purified fimbriae or fimbriae-bearing bacteria permitted precise quantitation of fimbrial antigen in cultures of strain CSH50, thereby providing a simple means of determining the effects of environmental conditions on the synthesis of type 1 fimbriae. | lld:pubmed |
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| pubmed-article:6128311 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6128311 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6128311 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6128311 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:6128311 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:6128311 | pubmed:author | pubmed-author:EisensteinB... | lld:pubmed |
| pubmed-article:6128311 | pubmed:author | pubmed-author:DoddD CDC | lld:pubmed |
| pubmed-article:6128311 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6128311 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:6128311 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6128311 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6128311 | pubmed:pagination | 764-73 | lld:pubmed |
| pubmed-article:6128311 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:6128311 | pubmed:meshHeading | pubmed-meshheading:6128311-... | lld:pubmed |
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| pubmed-article:6128311 | pubmed:meshHeading | pubmed-meshheading:6128311-... | lld:pubmed |
| pubmed-article:6128311 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6128311 | pubmed:articleTitle | Antigenic quantitation of type 1 fimbriae on the surface of Escherichia coli cells by an enzyme-linked immunosorbent inhibition assay. | lld:pubmed |
| pubmed-article:6128311 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6128311 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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