| pubmed-article:6121705 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C0814999 | lld:lifeskim |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C0007004 | lld:lifeskim |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C0017968 | lld:lifeskim |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C0023206 | lld:lifeskim |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C0022169 | lld:lifeskim |
| pubmed-article:6121705 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:6121705 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6121705 | pubmed:dateCreated | 1982-6-21 | lld:pubmed |
| pubmed-article:6121705 | pubmed:abstractText | The Thy-1 glycoprotein of mouse thymocytes was analysed with regard to the properties of its carbohydrate part. Of the different lectins tested, partial binding of Thy-1 from membrane extracts was found to Lens culinaris agglutinin (approximately 50%) and wheat germ agglutinin (approximately 25%). In contrast, concanavalin A bound all Thy-1. The results indicate that there is considerable microheterogeneity in the oligosaccaride chains within the Thy-1 population. Thy-1 was purified from thymocyte membrane extracts by concanavalin A affinity chromatography and gel filtration. The purified preparation revealed, on analysis by isoelectric focusing, at least six charge variants (isoelectric points ranging from approximately 5-9). These variants were isolated, treated with neuraminidase and tested for lectin binding and isoelectric point. It was shown that the charge heterogeneity was due to different amounts of sialic acid; even the most basic form contained at least one sialic acid residue. Furthermore, it was concluded that the degree of sialylation was not correlated to the lectin-binding properties. Analysis of Thy-1 from surface 125I-labelled thymocytes showed that the purified preparation was representative in its complexity of the Thy-1 glycoprotein exposed on the surface membrane. The possible biological implications of the findings are discussed. | lld:pubmed |
| pubmed-article:6121705 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6121705 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6121705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6121705 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6121705 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6121705 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:6121705 | pubmed:author | pubmed-author:StigbrandTT | lld:pubmed |
| pubmed-article:6121705 | pubmed:author | pubmed-author:CarlssonSS | lld:pubmed |
| pubmed-article:6121705 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6121705 | pubmed:volume | 123 | lld:pubmed |
| pubmed-article:6121705 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6121705 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6121705 | pubmed:pagination | 1-7 | lld:pubmed |
| pubmed-article:6121705 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:6121705 | pubmed:meshHeading | pubmed-meshheading:6121705-... | lld:pubmed |
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| pubmed-article:6121705 | pubmed:meshHeading | pubmed-meshheading:6121705-... | lld:pubmed |
| pubmed-article:6121705 | pubmed:meshHeading | pubmed-meshheading:6121705-... | lld:pubmed |
| pubmed-article:6121705 | pubmed:meshHeading | pubmed-meshheading:6121705-... | lld:pubmed |
| pubmed-article:6121705 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6121705 | pubmed:articleTitle | Carbohydrate complexity of the mouse thymocyte Thy-1 glycoprotein as demonstrated by lectin affinity and isoelectric focusing. | lld:pubmed |
| pubmed-article:6121705 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6121705 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6121705 | lld:pubmed |
