pubmed-article:6106502 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:6106502 | lifeskim:mentions | umls-concept:C1280551 | lld:lifeskim |
pubmed-article:6106502 | lifeskim:mentions | umls-concept:C0018787 | lld:lifeskim |
pubmed-article:6106502 | lifeskim:mentions | umls-concept:C0036208 | lld:lifeskim |
pubmed-article:6106502 | lifeskim:mentions | umls-concept:C2243361 | lld:lifeskim |
pubmed-article:6106502 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:6106502 | pubmed:dateCreated | 1980-12-18 | lld:pubmed |
pubmed-article:6106502 | pubmed:abstractText | An enriched fraction of plasma membranes was prepared from canine ventricle by a process which involved thorough disruption of membranes by vigorous homogenization in dilute suspension, sedimentation of contractile proteins and mitochondria at 3000 X g followed by sedimentation of a microsomal fraction at 200 000 X g. The microsomal suspension was then fractionated on a discontinuous sucrose gradient. Particles migrating in the density range 1.0591--1.1083 were characterized by (Na+ + K+)-ATPase activity and [3H]ouabain binding as being enriched in sarcolemma and were comprised of nonaggregated vesicles of diameter approx. 0.1 micron. These fractions contained (Ca2+ + Mg2+)-ATPase which appreared endogenous to the sarcolemma. The enzyme was solubilized using Triton X-100 and 1 M KCl and partially purified. Optimal Ca2+ concentration for enzyme activity was 5--10 microM. Both Na+ and K+ stimulated enzyme activity. It is suggested that the enzyme may be involved in the outward pumping of Ca2+ from the cardiac cell. | lld:pubmed |
pubmed-article:6106502 | pubmed:language | eng | lld:pubmed |
pubmed-article:6106502 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6106502 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:6106502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6106502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6106502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6106502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6106502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6106502 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:6106502 | pubmed:month | May | lld:pubmed |
pubmed-article:6106502 | pubmed:issn | 0006-3002 | lld:pubmed |
pubmed-article:6106502 | pubmed:author | pubmed-author:DrummondG IGI | lld:pubmed |
pubmed-article:6106502 | pubmed:author | pubmed-author:MorcosN CNC | lld:pubmed |
pubmed-article:6106502 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:6106502 | pubmed:day | 8 | lld:pubmed |
pubmed-article:6106502 | pubmed:volume | 598 | lld:pubmed |
pubmed-article:6106502 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:6106502 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:6106502 | pubmed:pagination | 27-39 | lld:pubmed |
pubmed-article:6106502 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
pubmed-article:6106502 | pubmed:meshHeading | pubmed-meshheading:6106502-... | lld:pubmed |
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pubmed-article:6106502 | pubmed:meshHeading | pubmed-meshheading:6106502-... | lld:pubmed |
pubmed-article:6106502 | pubmed:year | 1980 | lld:pubmed |
pubmed-article:6106502 | pubmed:articleTitle | (Ca2+ + Mg2+)-ATPase in enriched sarcolemma from dog heart. | lld:pubmed |
pubmed-article:6106502 | pubmed:publicationType | Journal Article | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6106502 | lld:pubmed |