| pubmed-article:6102477 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6102477 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:6102477 | lifeskim:mentions | umls-concept:C0206131 | lld:lifeskim |
| pubmed-article:6102477 | lifeskim:mentions | umls-concept:C0014239 | lld:lifeskim |
| pubmed-article:6102477 | lifeskim:mentions | umls-concept:C1148926 | lld:lifeskim |
| pubmed-article:6102477 | lifeskim:mentions | umls-concept:C2243361 | lld:lifeskim |
| pubmed-article:6102477 | lifeskim:mentions | umls-concept:C1159655 | lld:lifeskim |
| pubmed-article:6102477 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6102477 | pubmed:dateCreated | 1980-6-27 | lld:pubmed |
| pubmed-article:6102477 | pubmed:abstractText | Calcium uptake by adipocyte endoplasmic reticulum was studied in a rapidly obtained microsomal fraction. The kinetics and ionic requirements of Ca2+ transport in this preparation were characterized and compared to those of (Ca2+ + Mg2+)-ATPase activity. The time course of Ca2+ uptake in the presence of 5 mM oxalate was nonlinear, approaching a steady-state level of 10.8--11.5 nmol Ca2+/mg protein after 3--4 min of incubation. The rate of Ca2+ transport was iM oxalate. The calculated initial rate of calcium uptake was 18.5 nmol Ca2+/mg protein per min. The double reciprocal plot of ATP concentration against transport rate was nonlinear, with apparent Km values of 100 muM and 7 muM for ATP concentration ranges above and below 50 muM, respectively. The apparent Km values for Mg2+ and Ca2+ were 132 muM and 0.36--0.67 muM, respectively. The energy of activation was 23.4 kcal/mol. These kinetic properties were strikingly similar to those of the microsomal (Ca2+ + Mg2+)-ATPase. The presence of potassium was required for maximum Ca2+ transport activity. The order of effectiveness of monovalent cations in stimulating both Ca2+ transport and (Ca2+ + Mg2+)-ATPase activity was K+ greater than Na+ = NH4+ greater than Li+. Ca2+ transport and (Ca2+ + Mg2+)-ATPase activity were both inhibited 10--20% by 6 mM procaine and less than 10% by 10 mM sodium azide. Both processes were completely inhibited by 3 mM dibucaine or 50 muM p-chloromercuribenzene sulfonate. The results indicate that Ca2+ transport in adipocyte endoplasmic reticulum is mediated by a (Ca2+ + Mg2+)-ATPase and suggest an important role for endoplasmic reticulum in control of intracellular Ca2+ distribution. | lld:pubmed |
| pubmed-article:6102477 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6102477 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6102477 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6102477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6102477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6102477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6102477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6102477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6102477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6102477 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6102477 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6102477 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:6102477 | pubmed:author | pubmed-author:McDonaldJ MJM | lld:pubmed |
| pubmed-article:6102477 | pubmed:author | pubmed-author:JarettLL | lld:pubmed |
| pubmed-article:6102477 | pubmed:author | pubmed-author:BlackB LBL | lld:pubmed |
| pubmed-article:6102477 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6102477 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:6102477 | pubmed:volume | 596 | lld:pubmed |
| pubmed-article:6102477 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6102477 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6102477 | pubmed:pagination | 359-71 | lld:pubmed |
| pubmed-article:6102477 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:meshHeading | pubmed-meshheading:6102477-... | lld:pubmed |
| pubmed-article:6102477 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6102477 | pubmed:articleTitle | Relationship between calcium ion transport and (Ca2+ + Mg2+)-atpase activity in adipocyte endoplasmic reticulum. | lld:pubmed |
| pubmed-article:6102477 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6102477 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6102477 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6102477 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6102477 | lld:pubmed |
