| pubmed-article:6092488 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6092488 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:6092488 | lifeskim:mentions | umls-concept:C0017366 | lld:lifeskim |
| pubmed-article:6092488 | lifeskim:mentions | umls-concept:C0012893 | lld:lifeskim |
| pubmed-article:6092488 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:6092488 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:6092488 | pubmed:dateCreated | 1984-12-5 | lld:pubmed |
| pubmed-article:6092488 | pubmed:abstractText | Analysis of aminoglycoside-resistant Enterobacteriaceae isolated from patients at the Seattle Veterans Administration Medical Center indicated that a single 68-kilobase R factor was responsible for the epidemic spread of low-level resistance to gentamicin, kanamycin, and tobramycin. An examination, by means of the phosphocellulose paper binding assay, of resistant strains carrying this R factor resulted in the identification of a 2"-O-adenyltransferase [ANT(2")]-modifying enzyme. This enzyme was later detected in strains containing 150-kilobase plasmids. For more convenient monitoring of the dissemination of the ANT(2") gene among clinical isolates at the medical center, a DNA probe was developed by cloning of the ANT(2") structural gene from the 68-kilobase factor into pBR322. A 310-base pair Ava I restriction fragment isolated from the interior of the cloned ANT(2") gene was radiolabeled and used in Southern hybridization gels as a probe for plasmids isolated from aminoglycoside-resistant organisms. The probe proved to be highly specific and was more sensitive than enzymologic techniques for detection of the ANT(2") gene in clinical isolates with complex aminoglycoside resistance phenotypes. | lld:pubmed |
| pubmed-article:6092488 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6092488 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6092488 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6092488 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:6092488 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6092488 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:6092488 | pubmed:issn | 0022-1899 | lld:pubmed |
| pubmed-article:6092488 | pubmed:author | pubmed-author:YoungS ASA | lld:pubmed |
| pubmed-article:6092488 | pubmed:author | pubmed-author:PlordeJ JJJ | lld:pubmed |
| pubmed-article:6092488 | pubmed:author | pubmed-author:TompkinsL SLS | lld:pubmed |
| pubmed-article:6092488 | pubmed:author | pubmed-author:GouldN BNB | lld:pubmed |
| pubmed-article:6092488 | pubmed:author | pubmed-author:TenoverF CFC | lld:pubmed |
| pubmed-article:6092488 | pubmed:author | pubmed-author:GordonK PKP | lld:pubmed |
| pubmed-article:6092488 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6092488 | pubmed:volume | 150 | lld:pubmed |
| pubmed-article:6092488 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6092488 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6092488 | pubmed:pagination | 678-87 | lld:pubmed |
| pubmed-article:6092488 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:6092488 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6092488 | pubmed:articleTitle | Development of a DNA probe for the structural gene of the 2"-O-adenyltransferase aminoglycoside-modifying enzyme. | lld:pubmed |
| pubmed-article:6092488 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6092488 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6092488 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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