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| pubmed-article:6086955 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:6086955 | lifeskim:mentions | umls-concept:C0028630 | lld:lifeskim |
| pubmed-article:6086955 | lifeskim:mentions | umls-concept:C0439659 | lld:lifeskim |
| pubmed-article:6086955 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
| pubmed-article:6086955 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6086955 | pubmed:dateCreated | 1984-8-27 | lld:pubmed |
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| pubmed-article:6086955 | pubmed:abstractText | We have isolated a series of point mutants in the polyomavirus origin-intergenic control region by using a procedure which exploits the single-stranded nature of DNAs cloned into M13 phage both for the generation of mutants and for their analysis by DNA sequencing. In this report we describe the effects of cytosine X guanine----thymine X adenine base-pair substitutions in the polyomavirus origin region upon replication in mouse 3T6 cells of the M13-polyomavirus constructs. Our results indicate that sequences near the center of a 34-base-pair sequence with dyad symmetry are important for replication, whereas specific nucleotides near the ends of the dyad symmetry are not important. Furthermore, a putative large T antigen-binding site at nucleotides 60 to 80 can be mutated without affecting replication function as measured in this assay. | lld:pubmed |
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| pubmed-article:6086955 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6086955 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6086955 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6086955 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:6086955 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:6086955 | pubmed:author | pubmed-author:FolkW RWR | lld:pubmed |
| pubmed-article:6086955 | pubmed:author | pubmed-author:TriezenbergS... | lld:pubmed |
| pubmed-article:6086955 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6086955 | pubmed:volume | 51 | lld:pubmed |
| pubmed-article:6086955 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6086955 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6086955 | pubmed:pagination | 437-44 | lld:pubmed |
| pubmed-article:6086955 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6086955 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6086955 | pubmed:articleTitle | Essential nucleotides in the polyomavirus origin region. | lld:pubmed |
| pubmed-article:6086955 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6086955 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6086955 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:6086955 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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