| pubmed-article:6020412 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6020412 | lifeskim:mentions | umls-concept:C0728888 | lld:lifeskim |
| pubmed-article:6020412 | lifeskim:mentions | umls-concept:C0024467 | lld:lifeskim |
| pubmed-article:6020412 | lifeskim:mentions | umls-concept:C0038187 | lld:lifeskim |
| pubmed-article:6020412 | lifeskim:mentions | umls-concept:C0597299 | lld:lifeskim |
| pubmed-article:6020412 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6020412 | pubmed:dateCreated | 1967-5-10 | lld:pubmed |
| pubmed-article:6020412 | pubmed:abstractText | The metabolism of the ribosomal and soluble protein components of Aerobacter aerogenes was examined during its incubation in a Mg(++)-deficient medium. Bacteria were exposed to leucine-H(3) during the exponential growth period preceding Mg(++) starvation, and extracts were prepared after intervals of starvation and were centrifuged through gradients of sucrose to separate ribosomal from soluble proteins. Ribosomal proteins synthesized during the preceding exponential growth were slowly lost from the ribosomes; after 8 hr of starvation, few, if any, sedimented with ribosomes. Losses of total protein, together with the known rate of ribosome decay during Mg(++) starvation, suggested that these ribosomal proteins are ultimately degraded to acid-soluble products and account for all protein lost by the starving cells. These conclusions were supported by studies of Mg(++) starvation in a uracil-requiring strain of A. aerogenes: during uracil starvation a smaller fraction of the proteins synthesized were ribosomal, and the fraction of protein which subsequently decayed during Mg(++) starvation was correspondingly less. During recovery from Mg(++) starvation, proteins, lost from disintegrated ribosomes, were not detectably reutilized into new particles even before their degradation to acid-soluble products was complete. Synthesis of soluble proteins continued for more than 24 hr of starvation at a rate per milliliter close to 45% of the instantaneous rate per milliliter of the exponentially growing bacteria at the time Mg(++) was removed. This value agreed with that found previously for synthetic rates of deoxyribonucleic acid, transfer ribonucleic acid, and ribosomal ribonucleic acid during starvation relative to rates during exponential growth. | lld:pubmed |
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| pubmed-article:6020412 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6020412 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6020412 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6020412 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6020412 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:6020412 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:6020412 | pubmed:author | pubmed-author:KennellDD | lld:pubmed |
| pubmed-article:6020412 | pubmed:author | pubmed-author:MarchesiS LSL | lld:pubmed |
| pubmed-article:6020412 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6020412 | pubmed:volume | 93 | lld:pubmed |
| pubmed-article:6020412 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6020412 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6020412 | pubmed:pagination | 357-66 | lld:pubmed |
| pubmed-article:6020412 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6020412 | pubmed:year | 1967 | lld:pubmed |
| pubmed-article:6020412 | pubmed:articleTitle | Magnesium starvation of Aerobacter aerogenes. 3. Protein metabolism. | lld:pubmed |
| pubmed-article:6020412 | pubmed:publicationType | Journal Article | lld:pubmed |
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